Death Associated Protein 3 Recombinant Protein | DAP3 recombinant protein

Recombinant Death Associated Protein 3 (DAP3)

Cat. Num. AAA2123381

• Gene Names: DAP3; DAP-3; S29mt; MRPS29; MRP-S29; bMRP-10
• Applications: SDS-Page, Western Blot
• Purity: >97%
• Synonyms: Death Associated Protein 3; Recombinant Death Associated Protein 3 (DAP3); MRP-S29; MRPS29; bMRP-10; 28S Ribosomal Protein S29; Mitochondrial; Ionizing radiation resistance conferring protein; DAP3 recombinant protein

• Host: E Coli
• Purity/Purification: >97%
• Form/Format: Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Sequence Positions: Met1~Asn235
• Sequence Length: 398

• Applicable Applications for DAP3 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Source: Prokaryotic expression
• Species: Human
• Tag: N-terminal His Tag
• Subcellular Location: Secreted
• Isoelectric Point: 9.4
• Reconstitution: Reconstitute in PBS or others.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Product Categories/Family for DAP3 recombinant protein

Signal transduction

NCBI and Uniprot Product Information

• NCBI GI #: 1706299
• NCBI GeneID: 7818
• UniProt Accession #: P51398
• Molecular Weight: Predicted Molecular Mass: 30.9kDa; Accurate Molecular Mass: 31kDa
• NCBI Official Full Name: 28S ribosomal protein S29, mitochondrial
• NCBI Official Synonym Full Names: death associated protein 3
• NCBI Official Symbol: DAP3
• NCBI Official Synonym Symbols: DAP-3; S29mt; MRPS29; MRP-S29; bMRP-10
• NCBI Protein Information: 28S ribosomal protein S29, mitochondrial
• UniProt Protein Name: 28S ribosomal protein S29, mitochondrial
• Protein Family: 28S ribosomal protein
• UniProt Gene Name: DAP3
• UniProt Synonym Gene Names: MRPS29; MRP-S29; S29mt; DAP-3
• UniProt Entry Name: RT29_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq, Dec 2010]

Uniprot Description

DAP3: Involved in mediating interferon-gamma-induced cell death. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: nucleoplasm; small ribosomal subunit; mitochondrion; mitochondrial inner membrane; mitochondrial ribosome; nucleolus; nucleus

Molecular Function: protein binding

Biological Process: apoptotic mitochondrial changes; mitochondrial translation; organelle organization and biogenesis