Disabled Homolog 1 Recombinant Protein | DAB1 recombinant protein

Recombinant Disabled Homolog 1 (DAB1)

Cat. Num. AAA2029654

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Disabled Homolog 1; Recombinant Disabled Homolog 1 (DAB1); DAB1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; YKAKLIGIDEVSAARGDKL CQDSMMKLKG VVAGARSKGE HKQKIFLTIS FGGIKIFDEKTGALQHHHAV HEISYIAKDI TDHRAFGYVC GKEGNHRFVA IKTAQAAEPVILDLRDLFQL IYELKQRE
• Sequence Length: 555

• Applicable Applications for DAB1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 17.9kDa
• Accurate Molecular Mass (KD): 17kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Tyr42~Glu168
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 33350928
• NCBI GeneID: 1600
• NCBI Accession #: NP_066566.3
• NCBI GenBank Nucleotide #: NM_021080.3
• Molecular Weight: 59,950 Da
• NCBI Official Full Name: disabled homolog 1
• NCBI Official Synonym Full Names: Dab, reelin signal transducer, homolog 1 (Drosophila)
• NCBI Official Symbol: DAB1
• NCBI Protein Information: disabled homolog 1
• UniProt Protein Name: Disabled homolog 1
• Protein Family: Disabled
• UniProt Gene Name: DAB1
• UniProt Entry Name: DAB1_HUMAN

NCBI Description

The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. This gene is similar to disabled-1, and the protein encoded by this gene is thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

DAB1: an adaptor molecule functioning in neural development. The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. Thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. Associates with the SH2 domains of Src, Fyn and Abl. Phosphorylated in response to reelin induction in embryonic neurons

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p32-p31

Cellular Component: neuron projection; membrane; cell soma; apical part of cell; perinuclear region of cytoplasm; postsynaptic density; cytosol; brush border

Molecular Function: phosphoinositide 3-kinase binding

Biological Process: cell-cell adhesion involved in neuronal-glial interactions involved in cerebral cortex radial glia guided migration; response to drug; cerebellum structural organization; Golgi localization; ventral spinal cord development; negative regulation of cell adhesion; adult walking behavior; radial glia guided migration of Purkinje cell; small GTPase mediated signal transduction; positive regulation of protein kinase activity; negative regulation of astrocyte differentiation; dendrite development; midgut development; negative regulation of axonogenesis; cerebral cortex radially oriented cell migration; positive regulation of neuron differentiation; negative regulation of JAK-STAT cascade