Branched-chain-amino-acid aminotransferase-like protein 3, chloroplastic (At5g57850) Recombinant Protein | At5g57850 recombinant protein

Recombinant Arabidopsis thaliana Branched-chain-amino-acid aminotransferase-like protein 3, chloroplastic (At5g57850)

Cat. Num. AAA1391817

• Gene Names: ADCL; 4-amino-4-deoxychorismate lyase; MTI20.10; MTI20_10
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Branched-chain-amino-acid aminotransferase-like protein 3; chloroplastic (At5g57850); Recombinant Arabidopsis thaliana Branched-chain-amino-acid aminotransferase-like protein 3; At5g57850 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 58-373, Full length protein
• Sequence: SSYEVGERLKLARGGQQFLAMYSSVVDGITTDPAAMVLPLDDHMVHRGHGVFDTALIINGYLYELDQHLDRILRSASMAKIPLPFDRETIKRILIQTVSVSGCRDGSLRYWLSAGPGDFLLSPSQCLKPTLYAIVIKTNFAINPIGVKVVTSSIPIKPPEFATVKSVNYLPNVLSQMEAEAKGAYAGIWVCKDGFIAEGPNMNVAFVVNGGKELVMPRFDNVLSGCTAKRTLTLAEQLVSKGILKTVKVMDVTVEDGKKADEMMLIGSGIPIRPVIQWDEEFIGEGKEGPIAKALLDLLLEDMRSGPPSVRVLVPY
• Sequence Length: 316

• Species: Arabidopsis thaliana (Mouse-ear cress)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 1063741890
• NCBI GeneID: 835895
• NCBI Accession #: NP_001330897.1
• NCBI GenBank Nucleotide #: NM_001345276.1
• UniProt Accession #: Q8L493; Q9FJM9
• Molecular Weight: 41,065 Da
• NCBI Official Full Name: D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein
• NCBI Official Symbol: ADCL
• NCBI Official Synonym Symbols: 4-amino-4-deoxychorismate lyase; MTI20.10; MTI20_10
• NCBI Protein Information: D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein
• UniProt Protein Name: D-amino-acid transaminase, chloroplastic
• UniProt Gene Name: DAAT
• UniProt Synonym Gene Names: AtADCL

NCBI Description

ADCL encodes a protein that acts as a 4-amino-4-deoxychorismate lyase. It catalyzes the production 4-aminobenzoate (pABA) production which is required for folate biosynthesis. The enzyme localizes to chloroplasts based on an import assay and GFP localization experiments.

Uniprot Description

Amino acid aminotransferase showing activity for D-Asp and D-Ala as amino donors with 2-oxoglutarate as an amino acceptor. Can also use D-Met, D-Tyr, D-Phe, D-Gln, D-Trp and D-Asn as substrates, but no activity with L-Asp, L-Ala, L-Leu, L-Ile or L-Val. Catalyzes also the reverse reaction where an amino group is transferred from D-Glu to pyruvate or oxaloacetate to produce D-Ala or D-Asp, respectively. Also involved in folate biosynthesis, acting as an aminodeoxychorismate lyase converting 4-amino-4-deoxychorismate (ADC) to p-aminobenzoate (PABA)(PubMed:15500462).