Cytokeratin 19 Recombinant Protein

Cytokeratin 19, Recombinant, Human

Cat. Num. AAA637326

• Purity: Highly Purified; 95% by RP-HPLC and SDS-PAGE. Chromatographically purified.
• Synonyms: Cytokeratin 19; Recombinant; Human; Cytokeratin 19 recombinant protein

• Host: E Coli
• Purity/Purification: Highly Purified; 95% by RP-HPLC and SDS-PAGE. Chromatographically purified.
• Form/Format: Supplied as a lyophilized powder in 30mM Tris-HCl, pH 8.0, 9.5M urea, 2mM DTT, 2mM EDTA, 10mM methylammonium chloride. Reconstitute in sterile ddH2O, 0.1% BSA or HSA, to 0.1mg/ml, which can then be further diluted in assay buffer.

• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Cytokeratin 19 recombinant protein

Keratins are a class of fibrous proteins or scleroproteins important both as structural proteins and as keys to the study of protein conformation. The family represents the principal constituent of epidermis, hair, nails, horny tissues, and the organic matrix of tooth enamel. Two major conformational groups have been characterized, alpha-keratin, whose peptide backbone forms an alpha-helix, and beta-keratin, whose backbone forms a zigzag or pleated sheet structure. Cytokeratin 19 is a marker of simple epithelia. Cytokeratin 19 has been found in mesothelial and mesothelioma cells, and in ovarian cysts, cystadenomas, and ovarian carcinomas. It has been shown to be present in the basal layer of non-keratinizing stratified squamous epithelia such as the oral cavity and the ectocervix. Cytokeratin 19 has also been found in adenocarcinomas of the lung and in tumor cells of pulmonary metastases. It has been reported that cytokeratin 19 can be found in the ductal cells of normal pancreas and in pancreas cancers. It was found that ductal cells of normal pancreas express a number of cytokeratins including cytokeratin 19, whereas acinar cells of normal pancreas also express numerous cytokeratins but not cytokeratin 19. Thus it is determined that pancreas cancers consistently express certain cytokeratins including cytokeratin 19, as do normal ductal cells, which indicates that pancreas cancers may originate from normal ductal cells.

Recombinant human Cytokeratin 19 is a single, non-glycosylated polypeptide chain having a molecular mass of 44,098 Dalton.

Product Categories/Family for Cytokeratin 19 recombinant protein

Molecular Biology; MB-Keratin; Cytokeratin

NCBI and Uniprot Product Information

• NCBI GI #: 13537138
• Molecular Weight: 44kD
• NCBI Official Full Name: cytokeratin 19

Uniprot Description

K19: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 19 differs from all other IF proteins in lacking the C-terminal tail domain. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: dystrophin-associated glycoprotein complex; costamere; intermediate filament; Z disc; sarcolemma

Molecular Function: protein binding; structural constituent of cytoskeleton; structural constituent of muscle; protein complex binding

Biological Process: viral reproduction; response to estrogen stimulus; sarcomere organization