Cytohesin 4 Recombinant Protein | CYTH4 recombinant protein

Recombinant Cytohesin 4 (CYTH4)

Cat. Num. AAA2029343

• Gene Names: CYTH4; CYT4; PSCD4; DJ63G5.1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Cytohesin 4; Recombinant Cytohesin 4 (CYTH4); CYTH4 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MDLCHPEPAE LSSGETEELQ RIKWHRKQLL EDIQKLKDEI ADVFAQIDCFESAEESRMAQ KEKELCIGRK KFNMDPAKGI QYFIEHKLLT PDVQDIARFLYKGEGLNKTA IGTYLGERDP INLQVLQAFV DCHEFANLNL VQALRQFLWSFRLPGEAQKI DRMMEAFATR YCLCNPGVFQ STDTCYVLSF SIIMLNTSLHNPNVRDRPPF ERFVSMNRGI NNGSDLPEDQ LRNLFDSIKS EPFSIPEDDGNDLTHTFFNP DREGWLLKLG GRVKTWKRRW FILTDNCLYY FEFTTDKEPRGIIPLENLSV QKVDDPKKPF CLELYNPSCR GQKIKACKTD GDGRVVEGKHESYRISATSA EERDQWIESI RASITRVPF
• Sequence Length: 394

• Applicable Applications for CYTH4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 47.6kDa
• Accurate Molecular Mass (KD): 48kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal tags, his-tag and T7 tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Phe379
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 7019505
• NCBI GeneID: 27128
• NCBI Accession #: NP_037517.1
• NCBI GenBank Nucleotide #: NM_013385.3
• Molecular Weight: 45,672 Da
• NCBI Official Full Name: cytohesin-4
• NCBI Official Synonym Full Names: cytohesin 4
• NCBI Official Symbol: CYTH4
• NCBI Official Synonym Symbols: CYT4; PSCD4; DJ63G5.1
• NCBI Protein Information: cytohesin-4
• UniProt Protein Name: Cytohesin-4
• Protein Family: Cytohesin
• UniProt Gene Name: CYTH4
• UniProt Synonym Gene Names: CYT4; PSCD4
• UniProt Entry Name: CYH4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The encoded protein exhibits GEP activity in vitro with both ARF1 and ARF5 but is inactive with ARF6. The structures of this gene and CYTH1 are very similar. [provided by RefSeq, Aug 2008]

Uniprot Description

cytohesin 4: Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF through replacement of GDP with GTP.

Protein type: GEFs, ARF; GEFs

Chromosomal Location of Human Ortholog: 22q12.3-q13.1

Cellular Component: plasma membrane; trans-Golgi network

Molecular Function: lipid binding; ARF guanyl-nucleotide exchange factor activity

Biological Process: regulation of cell adhesion; vesicle-mediated transport; regulation of ARF protein signal transduction; positive regulation of GTPase activity