Mouse Cystatin C ELISA Kit | Cys-C elisa kit

Mouse Cystatin C ELISA Kit

Cat. Num. AAA761990

• Gene Names: CST3; ARMD11; HEL-S-2
• Reactivity: Mouse
• Synonyms: Cystatin C; Mouse Cystatin C ELISA Kit; Cystatin C/CST3/Cys-C/Cystatin 3/ARMD11/Post-Gamma-Globulin; Cys-C elisa kit

• Reactivity: Mouse
• Sequence Length: 146

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Cys-C elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Cystatin C antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Cystatin C antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Cystatin C amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Cystatin C can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 755738
• NCBI GeneID: 1471
• UniProt Accession #: P01034; Q6FGW9; B2R5J9; D3DW42
• Molecular Weight: 15,799 Da
• NCBI Official Full Name: cystatin C
• NCBI Official Synonym Full Names: cystatin C
• NCBI Official Symbol: CST3
• NCBI Official Synonym Symbols: ARMD11; HEL-S-2
• NCBI Protein Information: cystatin-C
• UniProt Protein Name: Cystatin-C
• UniProt Gene Name: CST3
• UniProt Entry Name: CYTC_HUMAN

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. In addition, this protein has been shown to have an antimicrobial function, inhibiting the replication of herpes simplex virus. Alternative splicing results in multiple transcript variants encoding a single protein. [provided by RefSeq, Nov 2014]

Uniprot Description

CST3: As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Defects in CST3 are the cause of amyloidosis type 6 (AMYL6); also known as hereditary cerebral hemorrhage with amyloidosis (HCHWA), cerebral amyloid angiopathy (CAA) or cerebroarterial amyloidosis Icelandic type. AMYL6 is a hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low. Genetic variations in CST3 are associated with age- related macular degeneration type 11 (ARMD11). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Belongs to the cystatin family.

Protein type: Secreted; Inhibitor; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: axon; basement membrane; cell soma; contractile fiber; endoplasmic reticulum; extracellular region; extracellular space; lysosome; multivesicular body; nuclear membrane; perinuclear region of cytoplasm

Molecular Function: beta-amyloid binding; cysteine protease inhibitor activity; endopeptidase inhibitor activity; identical protein binding; protease binding; protein binding

Biological Process: apoptosis; brain development; cell activation; cellular protein metabolic process; circadian sleep/wake cycle, REM sleep; defense response; embryo implantation; eye development; fibril organization and biogenesis; negative regulation of peptidase activity; negative regulation of proteolysis; positive regulation of cell proliferation; positive regulation of DNA replication; regulation of programmed cell death; regulation of tissue remodeling; response to axon injury; response to carbohydrate stimulus; response to drug; response to estradiol stimulus; response to hypoxia; response to nutrient levels; response to toxin; salivary gland development; Sertoli cell development

Disease: Cerebral Amyloid Angiopathy, Cst3-related; Macular Degeneration, Age-related, 11