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Testing Data #2 (Western blot analysis of extracts from HeLa cells and A549 cells, using Cytochrome P450 11A1 antibody.)

anti-Human Cytochrome P450 11A1 Antibody | anti-CYPXIA1 antibody

Cytochrome P450 11A1 Antibody

Gene Names
CYP11A1; CYP11A; CYPXIA1; P450SCC
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Cytochrome P450 11A1; Cytochrome P450 11A1 Antibody; Cholesterol side-chain cleavage enzyme mitochondrial; Cholesterol desmolase; Cytochrome P450(scc); CYP11A1; CYP11A; anti-CYPXIA1 antibody
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Cytochrome P450 11A1 antibody detects endogenous levels of total Cytochrome P450 11A1 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
521
Applicable Applications for anti-CYPXIA1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized peptide derived from C-terminal of human Cytochrome P450 11A1.
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data #2

(Western blot analysis of extracts from HeLa cells and A549 cells, using Cytochrome P450 11A1 antibody.)

Testing Data #2 (Western blot analysis of extracts from HeLa cells and A549 cells, using Cytochrome P450 11A1 antibody.)
References
Chung B.-C., Proc. Natl. Acad. Sci. U.S.A. 83:8962-8966(1986). Morohashi K., J. Biochem. 101:879-887(1987). Ota T., Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,152 Da
NCBI Official Full Name
cholesterol side-chain cleavage enzyme, mitochondrial isoform a
NCBI Official Synonym Full Names
cytochrome P450 family 11 subfamily A member 1
NCBI Official Symbol
CYP11A1
NCBI Official Synonym Symbols
CYP11A; CYPXIA1; P450SCC
NCBI Protein Information
cholesterol side-chain cleavage enzyme, mitochondrial
UniProt Protein Name
Cholesterol side-chain cleavage enzyme, mitochondrial
UniProt Gene Name
CYP11A1
UniProt Synonym Gene Names
CYP11A

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone, the precursor of most steroid hormones.

Research Articles on CYPXIA1

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Product Notes

The CYPXIA1 cyp11a1 (Catalog #AAA856572) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Cytochrome P450 11A1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 11A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:5000. Researchers should empirically determine the suitability of the CYPXIA1 cyp11a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 11A1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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