Rabbit Cyclophilin B ELISA Kit | CYPB elisa kit

Rabbit Cyclophilin B ELISA Kit

Cat. Num. AAA727723

• Reactivity: Rabbit
• Synonyms: Cyclophilin B; Rabbit Cyclophilin B ELISA Kit; CYPB elisa kit

• Reactivity: Rabbit

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 10-250ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This CYPB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit CYPB. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYPB elisa kit

Principle of the Assay: CYPB ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CYPB. Standards or samples are then added to the microtiter plate wells and CYPB if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CYPB present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for CYPB are added to each well to "sandwich" the CYPB immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CYPB and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYPB concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CYPB elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 2618851
• Molecular Weight: 45,473 Da
• NCBI Official Full Name: CypB
• UniProt Protein Name: Pulcherriminic acid synthase
• Protein Family: Bifunctional cytochrome P450/NADPH--P450 reductase
• UniProt Gene Name: cypX
• UniProt Synonym Gene Names: cyp134; cypB; BSU35060
• UniProt Entry Name: CYPX_BACSU

Uniprot Description

Involved in the biosynthesis of pulcherrimin, a red extracellular pigment. Catalyzes the oxidation of cyclo(L-Leu-L-Leu) (cLL) to yield pulcherriminic acid which forms pulcherrimin via a nonenzymic reaction with Fe3+. Substrates with small alkyl groups (cAA, cLG, cLP) exhibit weaker binding to CYP134A1, but substrates with larger hydrophobic side chains bind in a similar regime to cLL.