anti-Human Cytochrome P450 4F2 Antibody | anti-CYP4F2 antibody

Cytochrome P450 4F2 Antibody

Cat. Num. AAA8509824

• Gene Names: CYP4F2; CPF2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Cytochrome P450 4F2; Cytochrome P450 4F2 Antibody; EC 1.14.13.30; CYPIVF2; Leukotriene-B(4) omega-hydroxylase; Leukotriene-B(4) 20-monooxygenase; Cytochrome P450-LTB-omega; CP4F2; anti-CYP4F2 antibody

• Reactivity: Human
• Specificity: Cytochrome P450 4F2 antibody detects endogenous levels of total Cytochrome P450 4F2 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 520

• Applicable Applications for anti-CYP4F2 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:40000
• Immunogen: The antiserum was produced against synthesized peptide derived from N-terminal of human Cytochrome P450 4F2.
• Preparation and Storage: Store at-20 degree C for 1 year.

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using Cytochrome P450 4F2 antibody.)

References

Kikuta Y., FEBS Lett. 348:70-74(1994). Grimwood J., Nature 428:529-535(2004). Zhang X., Arch. Biochem. Biophys. 378:364-376(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 13435391
• NCBI GeneID: 8529
• NCBI Accession #: NP_001073.3
• NCBI GenBank Nucleotide #: NM_001082.4
• UniProt Accession #: P78329; Q16677; Q6NWT4; Q6NWT6; Q9NNZ0; Q9UIU8; A0A024R7K3; A8K425; B4DV75
• Molecular Weight: 21,709 Da
• NCBI Official Full Name: phylloquinone omega-hydroxylase CYP4F2
• NCBI Official Synonym Full Names: cytochrome P450 family 4 subfamily F member 2
• NCBI Official Symbol: CYP4F2
• NCBI Official Synonym Symbols: CPF2
• NCBI Protein Information: phylloquinone omega-hydroxylase CYP4F2
• UniProt Protein Name: Phylloquinone omega-hydroxylase CYP4F2
• Protein Family: Cytochrome
• UniProt Gene Name: CYP4F2
• UniProt Synonym Gene Names: 20-HETE synthase

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F11, is approximately 16 kb away. [provided by RefSeq, Jul 2008]

Uniprot Description

Omega-hydroxylase that oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids and xenobiotics. Plays a key role in vitamin K catabolism by mediating omega-hydroxylation of vitamin K1 (phylloquinone), and menaquinone-4 (MK-4), a form of vitamin K2. Hydroxylation of phylloquinone and MK-4 probably regulates blood coagulation (PubMed:19297519, PubMed:24138531). Also shows arachidonic acid omega-hydroxylase activity in kidney, by mediating conversion of arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE), possibly influencing blood pressure control (PubMed:10660572, PubMed:17341693, PubMed:18574070). Also acts as a leukotriene-B4 omega-hydroxylase by mediating conversion of leukotriene-B4 (LTB4) to its omega-hydroxylated metabolite 20-hydroxyleukotriene-B4 (20-OH LTB4) (PubMed:8026587, PubMed:9799565).