Cytochrome P450 3A2 (CYP3A2) Recombinant Protein | CYP3A2 recombinant protein

Recombinant Cytochrome P450 3A2 (CYP3A2)

Cat. Num. AAA2105332

• Gene Names: Cyp3a2; Cyp3a11
• Applications: SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Cytochrome P450 3A2 (CYP3A2); Recombinant Cytochrome P450 3A2 (CYP3A2); CYPIIIA2; Cytochrome P450/6-beta-A; Testosterone 6-beta-hydroxylase; Cytochrome P450-PCN2; CYP3A2 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic Expression
• Purity/Purification: > 90%
• Form/Format: Freeze-Dried Powder; PBS, pH7.4, Containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Sequence Positions: Met1~Asn280
• Sequence Length: 504

• Applicable Applications for CYP3A2 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Species: Rat
• Tag: N-terminal His-Tag
• Isoelectric Point: 9.4
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Subcellular Location: Secreted
• Reconstitution: Reconstitute in PBS or others.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Product Categories/Family for CYP3A2 recombinant protein

Human gene deletion

NCBI and Uniprot Product Information

• NCBI GI #: 31542329
• NCBI GeneID: 266682
• NCBI Accession #: NP_695224.2
• NCBI GenBank Nucleotide #: NM_153312.2
• UniProt Accession #: P05183
• Molecular Weight: Predicted MW: 36kDa; Accurate MW: 36kDa
• NCBI Official Full Name: cytochrome P450 3A2
• NCBI Official Synonym Full Names: cytochrome P450, family 3, subfamily a, polypeptide 2
• NCBI Official Symbol: Cyp3a2
• NCBI Official Synonym Symbols: Cyp3a11
• NCBI Protein Information: cytochrome P450 3A2
• UniProt Protein Name: Cytochrome P450 3A2
• Protein Family: Cytochrome
• UniProt Gene Name: Cyp3a2
• UniProt Synonym Gene Names: Cyp3a-2; Cyp3a11
• UniProt Entry Name: CP3A2_RAT

NCBI Description

catalyzes the conversion of testosterone to 6-beta-hydroxytestosterone [RGD, Feb 2006]

Uniprot Description

CYP3A4: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4- hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2- exo-monooxygenase. The enzyme also hydroxylates etoposide. Belongs to the cytochrome P450 family.

Protein type: Oxidoreductase; Endoplasmic reticulum; Membrane protein, peripheral; Cell surface; Demethylase; Xenobiotic Metabolism - metabolism by cytochrome P450; Cofactor and Vitamin Metabolism - retinol; Lipid Metabolism - linoleic acid; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Membrane protein, integral; EC 1.14.14.1; Xenobiotic Metabolism - drug metabolism - other enzymes

Cellular Component: endoplasmic reticulum membrane; cytoplasm

Molecular Function: enzyme binding; iron ion binding; heme binding; oxidoreductase activity; steroid hydroxylase activity; monooxygenase activity; steroid binding; demethylase activity; testosterone 6-beta-hydroxylase activity; vitamin D3 25-hydroxylase activity

Biological Process: steroid metabolic process; drug catabolic process; exogenous drug catabolic process; monoterpenoid metabolic process; alkaloid catabolic process; heterocycle metabolic process; drug metabolic process; steroid catabolic process