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Western Blot (WB) (Western blot analysis of extracts from HT-29 cells, using Cytochrome P450 2B6 antibody.)

anti-Human Cytochrome P450 2B6 Antibody | anti-CYP2B6 antibody

Cytochrome P450 2B6 Antibody

Gene Names
CYP2B6; CPB6; EFVM; IIB1; P450; CYP2B; CYP2B7; CYP2B7P; CYPIIB6
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Cytochrome P450 2B6; Cytochrome P450 2B6 Antibody; CP2B6; CPB1; CYP2B-1; CYPIIB1; CYPIIB6; Cytochrome P450 2B1; EC 1.14.14.1; P450 IIB1; P450-B; P450-LM2; P450-PB1; P450-PB2; anti-CYP2B6 antibody
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Cytochrome P450 2B6 antibody detects endogenous levels of total Cytochrome P450 2B6 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
491
Applicable Applications for anti-CYP2B6 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human Cytochrome P450 2B6.
Preparation and Storage
Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from HT-29 cells, using Cytochrome P450 2B6 antibody.)

Western Blot (WB) (Western blot analysis of extracts from HT-29 cells, using Cytochrome P450 2B6 antibody.)
References
Yamano S., Biochemistry 28:7340-7348(1989). Grimwood J., Nature 428:529-535(2004). Thum T., Lancet 355:979-983(2000).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,385 Da
NCBI Official Full Name
cytochrome P450 2B6
NCBI Official Synonym Full Names
cytochrome P450 family 2 subfamily B member 6
NCBI Official Symbol
CYP2B6
NCBI Official Synonym Symbols
CPB6; EFVM; IIB1; P450; CYP2B; CYP2B7; CYP2B7P; CYPIIB6
NCBI Protein Information
cytochrome P450 2B6
UniProt Protein Name
Cytochrome P450 2B6
Protein Family
UniProt Gene Name
CYP2B6

NCBI Description

This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008]

Uniprot Description

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,4-cineole 2-exo-monooxygenase.

Research Articles on CYP2B6

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Product Notes

The CYP2B6 cyp2b6 (Catalog #AAA851316) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Cytochrome P450 2B6 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 2B6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:1000. Researchers should empirically determine the suitability of the CYP2B6 cyp2b6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 2B6, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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