Human Cytochrome P450 2B1 ELISA Kit | CYP2B1 elisa kit

Human Cytochrome P450 2B1 ELISA Kit

Cat. Num. AAA744304

• Gene Names: Cyp2b1; Cypbe; Cyp2b-1
• Reactivity: Human
• Synonyms: Cytochrome P450 2B1; Human Cytochrome P450 2B1 ELISA Kit; CYP2B1 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYP2B1 elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This CYP2B1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CYP2B1. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY CYP2B1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CYP2B1 antibody and an CYP2B1-HRP conjugate. The assay sample and buffer are incubated together with CYP2B1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYP2B1 concentration since CYP2B1 from samples and CYP2B1-HRP conjugate compete for the anti-CYP2B1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYP2B1 from the sample, fewer sites are left to bind CYP2B1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP2B1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 198442825
• NCBI GeneID: 24300
• NCBI Accession #: NP_001128316.1
• NCBI GenBank Nucleotide #: NM_001134844.1
• Molecular Weight: 55,934 Da
• NCBI Official Full Name: cytochrome P450 2B1
• NCBI Official Synonym Full Names: cytochrome P450, family 2, subfamily b, polypeptide 1
• NCBI Official Symbol: Cyp2b1
• NCBI Official Synonym Symbols: Cypbe; Cyp2b-1
• NCBI Protein Information: cytochrome P450 2B1; P450b; P450-B; CYPIIB1; P450-LM2; cytochrome P450b; cytochrome P450-B; cytochrome P450-LM2; cytochrome P450-PB1; cytochrome P450-PB2; P450-PB1 and P450-PB2; Cytochrome P450, subfamily IIB (phenobarbital-inducible) (b, e)
• UniProt Protein Name: Cytochrome P450 2B1
• Protein Family: Cytochrome
• UniProt Gene Name: Cyp2b1
• UniProt Synonym Gene Names: Cyp2b-1; Cytochrome P450b
• UniProt Entry Name: CP2B1_RAT

NCBI Description

phenobarbitol-inducible member of the p450 xenobiotic-inducible superfamily involved in hydroxylation of decanoic and other fatty acids [RGD, Feb 2006]

Uniprot Description

CYP2B6: cytochrome P450, subfamily IIB. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Phosphorylation is accompanied by a decrease in enzyme activity.

Protein type: Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Oxidoreductase; Lipid Metabolism - arachidonic acid; EC 1.14.14.1

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm

Molecular Function: iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; arachidonic acid epoxygenase activity; steroid hydroxylase activity; oxygen binding

Biological Process: xenobiotic metabolic process; exogenous drug catabolic process; epoxygenase P450 pathway