Cytochrome b-245 Beta Polypeptide Recombinant Protein | CYBb recombinant protein

Recombinant Cytochrome b-245 Beta Polypeptide (CYBb)

Cat. Num. AAA2031367

• Gene Names: CYBB; CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Cytochrome b-245 Beta Polypeptide; Recombinant Cytochrome b-245 Beta Polypeptide (CYBb); CYBb recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; ERLVRFWR SQQKVVITKV VTHPFKTIEL QMKKKGFKME VGQYIFVKCP KVSKLEWHPF TLTSAPEEDF FSIHIRIVGD WTEGLFNACG CDKQEFQDAW KLPKIAVDGP FGTASEDVFS YEVVMLVGAG IGVTPFASIL KSVWYKYCNN ATNLKLKKIY FYWLCRDTHA FEWFADLLQL LESQMQERNN AGFLSYNIYL TGWDESQANH FAVHHDEEKD VITGLKQKTL YGRPNWDNEFKTIASQHPNT RIGVFLCGPE ALAETLSKQS ISNSESGPRG VHFIFNKENF
• Sequence Length: 570

• Applicable Applications for CYBb recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 36.9kDa
• Accurate Molecular Mass (KD): 35kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, T7 tag
• Organism Species: Homo sapiens (Human)
• Fragment: Pro399~Ala501
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119579857
• NCBI GeneID: 1536
• Molecular Weight: 65,336 Da
• NCBI Official Full Name: cytochrome b-245, beta polypeptide (chronic granulomatous disease)
• NCBI Official Synonym Full Names: cytochrome b-245, beta polypeptide
• NCBI Official Symbol: CYBB
• NCBI Official Synonym Symbols: CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
• NCBI Protein Information: cytochrome b-245 heavy chain
• UniProt Protein Name: Cytochrome b-245 heavy chain
• Protein Family: Cytochrome
• UniProt Gene Name: CYBB
• UniProt Synonym Gene Names: NOX2; Cytochrome b558 subunit beta
• UniProt Entry Name: CY24B_HUMAN

NCBI Description

Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]

Uniprot Description

CYBB: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of granulomatous disease,chronic, X-linked (CGD). A disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life- threatening bacterial/fungal infections. Defects in CYBB are a cause of mycobacteriosis atypical X-linked type 2 (AMCBX2). A rare condition characterized by predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections.

Protein type: Membrane protein, multi-pass; Mitochondrial; EC 1.-.-.-; Oxidoreductase; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp21.1

Cellular Component: Golgi apparatus; phagocytic vesicle membrane; rough endoplasmic reticulum; cell soma; mitochondrion; integral to plasma membrane; dendrite; plasma membrane; NADPH oxidase complex

Molecular Function: protein binding; electron carrier activity; FAD binding; protein heterodimerization activity; superoxide-generating NADPH oxidase activity; metal ion binding; heme binding; voltage-gated ion channel activity

Biological Process: response to drug; respiratory burst; interaction with host; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; ion transport; vascular endothelial growth factor receptor signaling pathway; inflammatory response; superoxide release; response to nutrient; hydrogen peroxide biosynthetic process

Disease: Immunodeficiency 34; Granulomatous Disease, Chronic, X-linked