Typical Testing Data/Standard Curve (for reference only)
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Mouse CXC-chemokine receptor 2 ELISA Kit | CXCR2 elisa kit
Mouse CXC-chemokine receptor 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
()
Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: CXCR2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CXCR2 antibody and an CXCR2-HRP conjugate. The assay sample and buffer are incubated together with CXCR2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CXCR2 concentration since CXCR2 from samples and CXCR2-HRP conjugate compete for the anti-CXCR2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CXCR2 from the sample, fewer sites are left to bind CXCR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CXCR2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009]
Uniprot Description
IL-8R B: Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, integral; Receptor, GPCR; Receptor, cytokine; Membrane protein, multi-pass; GPCR, family 1
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: mast cell granule; cell surface; membrane; integral to plasma membrane; plasma membrane; intracellular
Molecular Function: protein binding; signal transducer activity; interleukin-8 receptor activity; interleukin-8 binding; C-X-C chemokine receptor activity
Biological Process: neutrophil chemotaxis; neutrophil activation; signal transduction; chemotaxis; positive regulation of vascular permeability; negative regulation of neutrophil apoptosis; positive regulation of angiogenesis; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; receptor internalization; positive regulation of cell proliferation; midbrain development; cellular defense response; dendritic cell chemotaxis; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); inflammatory response; acute inflammatory response to antigenic stimulus
Research Articles on CXCR2
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Product Notes
The Mouse CXCR2 cxcr2 (Catalog #AAA726530) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA726530 ELISA Kit recognizes Mouse CXCR2. It is sometimes possible for the material contained within the vial of "CXC-chemokine receptor 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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