Typical Testing Data/Standard Curve (for reference only)
Human CXC chemokine receptor 2 ELISA Kit | CXCR2 elisa kit
Human CXC chemokine receptor 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This CXCR2 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forCXCR2. Standards or samples are then added to the microtiter plate wells andCXCR2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofCXCR2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forCXCR2 are added to each well to "sandwich" theCXCR2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containCXCR2 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009]
Uniprot Description
IL-8R B: Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Receptor, cytokine
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: cell surface; mast cell granule; membrane; integral to plasma membrane; plasma membrane; intracellular
Molecular Function: protein binding; signal transducer activity; interleukin-8 receptor activity; interleukin-8 binding; C-X-C chemokine receptor activity
Biological Process: neutrophil chemotaxis; neutrophil activation; signal transduction; chemotaxis; positive regulation of vascular permeability; negative regulation of neutrophil apoptosis; elevation of cytosolic calcium ion concentration; positive regulation of angiogenesis; cell surface receptor linked signal transduction; receptor internalization; positive regulation of cell proliferation; midbrain development; dendritic cell chemotaxis; cellular defense response; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); inflammatory response; acute inflammatory response to antigenic stimulus
Research Articles on CXCR2
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Product Notes
The Human CXCR2 cxcr2 (Catalog #AAA727762) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA727762 ELISA Kit recognizes Human CXCR2. It is sometimes possible for the material contained within the vial of "CXC chemokine receptor 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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