CXCL14 recombinant protein

Recombinant Human CXCL14 Protein

Cat. Num. AAA8248905

• Gene Names: CXCL14; KEC; KS1; BMAC; BRAK; NJAC; MIP2G; MIP-2g; SCYB14
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Synonyms: CXCL14; Recombinant Human CXCL14 Protein; MIP2G; NJAC; SCYB14; C-X-C motif chemokine 14; Chemokine BRAK; MIP-2G; Small-inducible cytokine B14; CXCL14 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM Tris-HCl, 1M NaCl, pH 8.5.
• Sequence: SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIITTKS VSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE
• Sequence Length: 111

• Applicable Applications for CXCL14 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for CXCL14 recombinant protein

Recombinant Human CXCL14 Protein is produced by E Coli. It is a single non-glycosylated polypeptide chain containing 77 amino acids with a molecular mass of 9.4 kD.

NCBI and Uniprot Product Information

• NCBI GI #: 20149565
• NCBI GeneID: 9547
• NCBI Accession #: NP_004878.2
• NCBI GenBank Nucleotide #: NM_004887.4
• UniProt Accession #: O95715; Q6UW97; Q86U69; Q9BTR1; Q9NS21; B3KQU8
• Molecular Weight: 13,078 Da
• NCBI Official Full Name: C-X-C motif chemokine 14
• NCBI Official Synonym Full Names: C-X-C motif chemokine ligand 14
• NCBI Official Symbol: CXCL14
• NCBI Official Synonym Symbols: KEC; KS1; BMAC; BRAK; NJAC; MIP2G; MIP-2g; SCYB14
• NCBI Protein Information: C-X-C motif chemokine 14
• UniProt Protein Name: C-X-C motif chemokine 14
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: CXCL14
• UniProt Synonym Gene Names: MIP2G; NJAC; SCYB14
• UniProt Entry Name: CXL14_HUMAN

NCBI Description

This antimicrobial gene belongs to the cytokine gene family which encode secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages. It has been implicated that this cytokine is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL14: Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: Golgi apparatus

Molecular Function: protein binding

Biological Process: cell-cell signaling; chemotaxis; signal transduction