SDF-1 ALPHA recombinant protein
RECOMBINANT HUMAN SDF-1 ALPHA
Functional Assays: Minimum Dilution: 20ng/ml; Maximum Dilution: 80ng/ml;
Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
Preparation
Shelf Life: 3 months from date of reconstitution.
NCBI and Uniprot Product Information
NCBI Description
This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
Uniprot Description
CXCL12: Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to another C-X-C chemokine receptor CXCR7, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1- alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3- 67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and CXCR7, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T- cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of CXCR7 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Monomer or homodimer; in equilibrium. Dimer formation is induced by non acidic pH and the presence of multivalent anions, and by binding to CXCR4 or heparin. Monomeric form is required for full chemotactic activity and resistance to ischemia/reperfusion injury, whereas the dimeric form acts as a partial agonist of CXCR4, stimulating Ca2+ mobilization but with no chemotactic activity and instead acts as a selective antagonist that blocks chemotaxis induced by the monomeric form. Interacts with the N- terminus of CXCR7. Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen. Belongs to the intercrine alpha (chemokine CxC) family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Chemokine; Motility/polarity/chemotaxis; Secreted; Cell development/differentiation
Chromosomal Location of Human Ortholog: 10q11.1
Cellular Component: extracellular space; extracellular region; external side of plasma membrane
Molecular Function: growth factor activity; chemokine activity; chemokine receptor binding; CXCR chemokine receptor binding; receptor binding
Biological Process: response to peptide hormone stimulus; positive regulation of dopamine secretion; positive regulation of cell adhesion; blood circulation; adult locomotory behavior; neuron migration; motor axon guidance; signal transduction; chemotaxis; induction of positive chemotaxis; germ cell development; T cell proliferation; response to radiation; germ cell migration; cell adhesion; organ regeneration; response to virus; regulation of actin polymerization and/or depolymerization; cellular calcium ion homeostasis; patterning of blood vessels; G-protein coupled receptor protein signaling pathway; response to mechanical stimulus; response to heat; ameboidal cell migration; positive regulation of axon extension involved in axon guidance; response to hypoxia; positive regulation of endothelial cell proliferation; immune response; telencephalon cell migration
Disease: Human Immunodeficiency Virus Type 1, Susceptibility To
Research Articles on SDF-1 ALPHA
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Product Notes
The SDF-1 ALPHA cxcl12 (Catalog #AAA232302) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SDF-1 ALPHA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Functional Assays (FN), Western Blot (WB). ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well; Functional Assays: Minimum Dilution: 20ng/ml; Maximum Dilution: 80ng/ml; Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane. Researchers should empirically determine the suitability of the SDF-1 ALPHA cxcl12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SDF-1 ALPHA, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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