Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) Recombinant Protein | ITaC recombinant protein

Recombinant Interferon Inducible T-Cell Alpha Chemoattractant (ITaC)

Cat. Num. AAA2011208

• Gene Names: CXCL11; IP9; H174; IP-9; b-R1; I-TAC; SCYB11; SCYB9B
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Interferon Inducible T-Cell Alpha Chemoattractant (ITaC); Recombinant Interferon Inducible T-Cell Alpha Chemoattractant (ITaC); ITaC recombinant protein

• Host: E Coli
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- FPMFKRGRC LCIGPGVKAV KVADIEKASI MYPSNNCDKI EVIITLKENK GQRCLNPKSK QARLIIKKVE RKNF
• Sequence Length: 94

• Applicable Applications for ITaC recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Phe22~Phe94 (Accession # O14625) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4885589
• NCBI GeneID: 6373
• NCBI Accession #: NP_005400.1
• NCBI GenBank Nucleotide #: NM_005409.4
• UniProt Accession #: O14625; Q53YA3; Q92840
• Molecular Weight: 12.1kDa
• NCBI Official Full Name: C-X-C motif chemokine 11
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 11
• NCBI Official Symbol: CXCL11
• NCBI Official Synonym Symbols: IP9; H174; IP-9; b-R1; I-TAC; SCYB11; SCYB9B
• NCBI Protein Information: C-X-C motif chemokine 11; beta-R1; small inducible cytokine B11; small-inducible cytokine B11; interferon gamma-inducible protein 9; interferon-inducible T-cell alpha chemoattractant; small inducible cytokine subfamily B (Cys-X-Cys), member 11; small inducible cytokine subfamily B (Cys-X-Cys), member 9B
• UniProt Protein Name: C-X-C motif chemokine 11
• UniProt Gene Name: CXCL11
• UniProt Synonym Gene Names: ITAC; SCYB11; SCYB9B; IP-9; I-TAC
• UniProt Entry Name: CXL11_HUMAN

NCBI Description

Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC. This antimicrobial gene is a CXC member of the chemokine superfamily. Its encoded protein induces a chemotactic response in activated T-cells and is the dominant ligand for CXC receptor-3. The gene encoding this protein contains 4 exons and at least three polyadenylation signals which might reflect cell-specific regulation of expression. IFN-gamma is a potent inducer of transcription of this gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

Uniprot Description

CXCL11: Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Chemokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q21.2

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; protein binding; CXCR3 chemokine receptor binding; chemokine activity

Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; immune response; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of cAMP metabolic process; inflammatory response; chemotaxis; signal transduction; regulation of cell proliferation