Human Connexin 37 ELISA Kit | CX37 elisa kit

Human Connexin 37 ELISA Kit

Cat. Num. AAA726273

• Gene Names: GJA4; CX37
• Reactivity: Human
• Synonyms: Connexin 37; Human Connexin 37 ELISA Kit; CX37 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CX37 elisa kit

For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

Intended Uses: This CX37 ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of CX37 in the sample, this CX37 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus CX37 concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of CX37 utilizes a multiclonal anti-CX37 antibody and an CX37-HRP conjugate. The assay sample and buffer are incubated together with CX37-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CX37 concentration since CX37 from samples and CX37-HRP conjugate compete for the anti-CX37 antibody binding site. Since the number of sites is limited, as more sites are occupied by CX37 from the sample, fewer sites are left to bind CX37-HRP conjugate. Standards of known CX37 concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of CX37. The CX37 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CX37 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 7416865
• NCBI GeneID: 2701
• Molecular Weight: 37,414 Da
• NCBI Official Full Name: connexin 37
• NCBI Official Synonym Full Names: gap junction protein, alpha 4, 37kDa
• NCBI Official Symbol: GJA4
• NCBI Official Synonym Symbols: CX37
• NCBI Protein Information: gap junction alpha-4 protein; connexin 37; connexin-37
• UniProt Protein Name: Gap junction alpha-4 protein
• UniProt Gene Name: GJA4
• UniProt Synonym Gene Names: Cx37
• UniProt Entry Name: CXA4_HUMAN

NCBI Description

This gene encodes a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene have been associated with atherosclerosis and a higher risk of myocardial infarction. [provided by RefSeq, Jul 2008]

Uniprot Description

GJA4: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Belongs to the connexin family. Alpha-type (group II) subfamily.

Protein type: Cell adhesion; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: connexon complex; integral to plasma membrane; gap junction

Molecular Function: protein binding

Biological Process: intercellular junction assembly; blood vessel development; cell-cell signaling; transport; calcium ion transport; response to pain