Russell Double Sugar Agar Culture Media
Russell Double Sugar Agar (Powder)
Purity
Microbiological Grade
Synonyms
Russell Double Sugar Agar; Russell Double Sugar Agar (Powder); Russell Double Sugar Agar culture media
Purity/Purification
Microbiological Grade
Preparation and Storage
Store the sealed bottle containing the dehydrated medium at 2 to 30 degree C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color to pink color.
Related Product Information for Russell Double Sugar Agar culture media
Russell Double Sugar Agar is a differential agar medium used for the identification of enteric bacteria based on the fermentation of dextrose and lactose with or without gas production.
The original formula of Russell Double Sugar Agar developed by Russell was modified by Nicols by replacing litmus indicator with phenol red. Selected colonies are transferred from the primary plating medium to tubes of Russell Double Sugar Agar using a straight bacteriological wire, stabbing the butt of the tube and streaking the slanted portion of the medium. Inoculated tubes after incubation are observed for pH reactions and gas formation. Phenol red indicates fermentation of the carbohydrates by the production of a yellow color (acid reaction); lack of fermentation is indicated by a red color (alkaline reaction). Gas formation is indicated by the presence of bubbles or cracks in the medium. After 24-48 hours incubation under aerobic conditions, a tube with a red slant and yellow butt indicates dextrose fermentation. Lactose fermentation is indicated by a yellow slant and butt. A red slant and butt indicates no fermentation. Other biochemical or serological tests are performed as required to confirm identification.
The original formula of Russell Double Sugar Agar developed by Russell was modified by Nicols by replacing litmus indicator with phenol red. Selected colonies are transferred from the primary plating medium to tubes of Russell Double Sugar Agar using a straight bacteriological wire, stabbing the butt of the tube and streaking the slanted portion of the medium. Inoculated tubes after incubation are observed for pH reactions and gas formation. Phenol red indicates fermentation of the carbohydrates by the production of a yellow color (acid reaction); lack of fermentation is indicated by a red color (alkaline reaction). Gas formation is indicated by the presence of bubbles or cracks in the medium. After 24-48 hours incubation under aerobic conditions, a tube with a red slant and yellow butt indicates dextrose fermentation. Lactose fermentation is indicated by a yellow slant and butt. A red slant and butt indicates no fermentation. Other biochemical or serological tests are performed as required to confirm identification.
Product Categories/Family for Russell Double Sugar Agar culture media
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Product Notes
The Russell Double Sugar Agar (Catalog #AAA653062) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Russell Double Sugar Agar, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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