Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base Culture Media
Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base (Powder)
Purity
Molecular Biology Grade
Synonyms
Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base; Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base (Powder); Synthetic complete amino acid supplement-minus uracil (UraDO); Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base culture media
Purity/Purification
Molecular Biology Grade
Form/Format
White, homogeneous, free flowing powder
Solubility
Colorless, clear, complete
pH
4.2
Directions per Liter (Broth)
Mix 1.92 grams of drop-out mix with 6.7g MBS653287 Yeast Nitrogen Base w/o AA, Carbohydrate & w/ AS per liter of ddH2O. Adjust to desired pH. Sterilize with 0.2 um membrane filter. Aliquot into sterile containers.
Directions per Liter (Plates)
Mix 1.92 grams of drop-out mix with 43.7 g MBS653105 Drop-out Base (w/o aa) With Glucose and Agar per liter of ddH2O. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121°C (15 psi).
Formulation
Each bottle is a homogenous mixture of the following amino acids and nucleotide bases minus uracil:
Amino Acids:
p-Aminobenzoic acid: 0.2g
Alanine: 2.0g
Arginine: 2.0g
Asparagine: 2.0g
Aspartic acid: 2.0g
Cysteine: 2.0g
Glutamic acid: 2.0g
Glutamine: 2.0g
Glycine: 2.0g
Histidine: 2.0g
Isoleucine: 2.0g
Leucine: 10.0g
Lysine: 2.0g
Methionine: 2.0g
Phenylalanine: 2.0g
Proline: 2.0g
Serine: 2.0g
Threonine: 2.0g
Tryptophan: 2.0g
Tyrosine: 2.0g
Valine: 2.0g
Nucleotide Bases:
Adenine: 0.5g
Vitamins:
Inositol: 2.0g
Uracil: Absent
Use 1.92g/liter
100g makes ~50 liters
Amino Acids:
p-Aminobenzoic acid: 0.2g
Alanine: 2.0g
Arginine: 2.0g
Asparagine: 2.0g
Aspartic acid: 2.0g
Cysteine: 2.0g
Glutamic acid: 2.0g
Glutamine: 2.0g
Glycine: 2.0g
Histidine: 2.0g
Isoleucine: 2.0g
Leucine: 10.0g
Lysine: 2.0g
Methionine: 2.0g
Phenylalanine: 2.0g
Proline: 2.0g
Serine: 2.0g
Threonine: 2.0g
Tryptophan: 2.0g
Tyrosine: 2.0g
Valine: 2.0g
Nucleotide Bases:
Adenine: 0.5g
Vitamins:
Inositol: 2.0g
Uracil: Absent
Use 1.92g/liter
100g makes ~50 liters
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Related Product Information for Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base culture media
Drop-out Mix Synthetic-minus Uracil is used as an addition to Drop-out Base for developing synthetic, drop-out media formulations for selecting auxotrophic cells.
Product Categories/Family for Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base culture media
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Product Notes
The Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base (Catalog #AAA652948) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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