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Brain Heart Infusion Broth w/o Dextrose Culture Media

Brain Heart Infusion Broth w/o Dextrose (Powder)

Synonyms
Brain Heart Infusion Broth w/o Dextrose; Brain Heart Infusion Broth w/o Dextrose (Powder); Brain Heart Infusion Broth w/o Dextrose culture media
Ordering
 
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For Research Use Only!
Appearance
Beige homogeneous, free flowing powder
Solubility
Light amber, clear and complete
pH
7.3
Microbiological Analysis
Aspergillus niger # 16404: Very Good
Candioa albicans # 10231: Excellent
Enterococcus faecalis # 29212: Excellent
Escherichia coli # 25922: Excellent
Neisseria meningitides # 13090: Good
Pseudomonas aeruginosa # 27853: Excellent
Saccharomyces cerevisiae # 9763: Very Good
Staphylococcus aureus # 25923: Excellent
Streptococcus pneumoniae # 6305: Good
Streptococcus pyogenes # 19615: Excellent
Directions per Liter
Dissolve 35g in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121°C (15psi).
BHI Broth
Components shown as g/liter:
- Brain Heart Infusion from Solids: 17.5
- Disodium Phosphate: 2.5
- Pancreatic Digest of Gelatin: 10.0
- Sodium Chloride: 5.0

Total: 35g/liter
Preparation and Storage
Store powdered media at RT/4°C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
Related Product Information for Brain Heart Infusion Broth w/o Dextrose culture media
Brain Heart Infusion Broth without Dextrose is a modification of the medium described by Rosenow and Hayden In which infusion from calf brains has replaced the brain tissue and disodium phosphate has replaced the calcium carbonate buffer. The addition of 0.1 % agar can be used to lower oxygen tension to enhance the growth of anaerobic organisms. Brain Heart Infusion Broth without Dextrose is a basal medium used with added carbohydrates for fermentation studies.

For the cultivation of fastidious aerobic and anaerobic bacteria.
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Product Notes

The Brain Heart Infusion Broth w/o Dextrose (Catalog #AAA652861) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Brain Heart Infusion Broth w/o Dextrose, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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