Rat Cathepsin S ELISA Kit | CTSS elisa kit

Rat Cathepsin S ELISA Kit

Cat. Num. AAA11479

• Reactivity: Rat
• Synonyms: Cathepsin S; Rat Cathepsin S ELISA Kit; CTSS elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Rat CTSS and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 3.12 ng/ml - 100 ng/ml.
• Sensitivity: 1.0 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated CTSS concentrations in Rat serum, plasma and other body fluids. Using Purified Rat CTSS antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CTSS and CTSS antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CTSS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for CTSS elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated CTSS concentrations in Rat serum, plasma and other body fluids. Using Purified Rat CTSS antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CTSS and CTSS antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CTSS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 315075311
• NCBI GeneID: 1520
• NCBI Accession #: NP_001186668.1
• NCBI GenBank Nucleotide #: NM_001199739.1
• UniProt Accession #: P25774; Q5T5I0; Q6FHS5; Q9BUG3; B4DWC9; D3DV05
• Molecular Weight: 37,496 Da
• NCBI Official Full Name: cathepsin S isoform 2 preproprotein
• NCBI Official Synonym Full Names: cathepsin S
• NCBI Official Symbol: CTSS
• NCBI Protein Information: cathepsin S
• UniProt Protein Name: Cathepsin S
• Protein Family: Cathepsin
• UniProt Gene Name: CTSS
• UniProt Entry Name: CATS_HUMAN

NCBI Description

The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

CTSS: Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond- specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N. Belongs to the peptidase C1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.27; Protease

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; lysosomal lumen; intracellular membrane-bound organelle; membrane; lysosome; extracellular region

Molecular Function: collagen binding; proteoglycan binding; fibronectin binding; cysteine-type endopeptidase activity; laminin binding

Biological Process: extracellular matrix organization and biogenesis; adaptive immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis; antigen processing and presentation; extracellular matrix disassembly; collagen catabolic process; antigen processing and presentation of peptide antigen via MHC class I; proteolysis involved in cellular protein catabolic process; toll-like receptor signaling pathway; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response; positive regulation of inflammatory response