CTSH recombinant protein

Recombinant Human CTSH Protein

Cat. Num. AAA8248884

• Gene Names: CTSH; ACC4; ACC5; CPSB; ACC-4; ACC-5
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: CTSH; Recombinant Human CTSH Protein; CPSB; Pro-cathepsin H; CTSH recombinant protein

• Host: Human cells
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Sequence: AELSVNSLEK FHFKSWMSKH RKTYSTEEYH HRLQTFASNW RKINAHNNGN HTFKMALNQF SDMSFAEIKH KYLWSEPQNC SATKSNYLRG TGPYPPSVDW RKKGNFVSPV KNQGACGSCW TFSTTGALES AIAIATGKML SLAEQQLVDC AQDFNNHGCQ GGLPSQAFEY ILYNKGIMGE DTYPYQGKDG YCKFQPGKAI GFVKDVANIT IYDEEAMVEA VALYNPVSFA FEVTQDFMMY RTGIYSSTSC HKTPDKVNHA VLAVGYGEKN GIPYWIVKNS WGPQWGMNGY FLIERGKNMC GLAACASYPI PLVVDHHHHH H
• Sequence Length: 335

• Applicable Applications for CTSH recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for CTSH recombinant protein

Recombinant Human CTSH Protein is produced by our mammalian expression system and the target gene encoding Ala23-Val335 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 23110955
• NCBI GeneID: 1512
• NCBI Accession #: NP_004381.2
• NCBI GenBank Nucleotide #: NM_004390.4
• UniProt Accession #: P09668; Q96NY6; Q9BUM7; B2RBK0
• Molecular Weight: 37,394 Da
• NCBI Official Full Name: pro-cathepsin H isoform a preproprotein
• NCBI Official Synonym Full Names: cathepsin H
• NCBI Official Symbol: CTSH
• NCBI Official Synonym Symbols: ACC4; ACC5; CPSB; ACC-4; ACC-5
• NCBI Protein Information: pro-cathepsin H
• UniProt Protein Name: Pro-cathepsin H
• Protein Family: Pro-cathepsin
• UniProt Gene Name: CTSH
• UniProt Synonym Gene Names: CPSB
• UniProt Entry Name: CATH_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

Uniprot Description

CTSH: Important for the overall degradation of proteins in lysosomes. Belongs to the peptidase C1 family.

Protein type: EC 3.4.22.16; Protease

Chromosomal Location of Human Ortholog: 15q25.1

Cellular Component: cytosol; extracellular space; lysosome

Molecular Function: aminopeptidase activity; caspase activator activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; endopeptidase activity; HLA-A specific activating MHC class I receptor activity; peptidase activity; protein binding; serine-type endopeptidase activity

Biological Process: adaptive immune response; antigen processing and presentation; cellular protein metabolic process; immune response-regulating signal transduction; membrane protein proteolysis; metanephros development; negative regulation of apoptosis; positive regulation of angiogenesis; positive regulation of cell migration; positive regulation of cell proliferation; protein destabilization; proteolysis; proteolysis involved in cellular protein catabolic process; response to retinoic acid; surfactant homeostasis; T cell mediated cytotoxicity; zymogen activation