Troponin I Cardiac Protein | cTnI protein
Human cardiac troponin I (cTnI)
Synonyms
Troponin I Cardiac; Human cardiac troponin I (cTnI); cTnI protein
Form/Format
Freeze-dried from 0.01 M HCl.
Product is recommended to reconstitute with urea/Tris buffer, pH 7.5 (7 M urea, 5 mM EDTA, 15 mM
mercapthoethanol, 20 mM Tris) to concentration around 1mg/ml.
Solubility is limited at neutral pH and physiolog
Product is recommended to reconstitute with urea/Tris buffer, pH 7.5 (7 M urea, 5 mM EDTA, 15 mM
mercapthoethanol, 20 mM Tris) to concentration around 1mg/ml.
Solubility is limited at neutral pH and physiolog
Sequence Length
211
Application Notes
TnI is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium sensitivity to striated muscle actomyosin ATPase activity.
TnI exists in three isoforms, one for slow-twitch skeletal muscle, one for fast twitch skeletal muscle and one for cardiac muscle. The amino acid sequences of two skeletal (skTnI) and one cTnI forms how 40% dissimilarity. Furthermore, the human cTnI has additional amino acid residues on its N-terminal region that are not present on the skeletal form, making this protein a promising analyte for indicating cardiac specificity. cTnI follows similar release pattern as CK-MB within the first 15 hours of mydegree Cardial infarction (MI), but remains elevated in serum up to 6 days. This observation and the unique amino sequence of cTnI make it an important marker of MI.
TnI is suitable for use as a standard in immunoassay for early detection of MI, immunogen for antiserum production and tracer for iodination.
TnI exists in three isoforms, one for slow-twitch skeletal muscle, one for fast twitch skeletal muscle and one for cardiac muscle. The amino acid sequences of two skeletal (skTnI) and one cTnI forms how 40% dissimilarity. Furthermore, the human cTnI has additional amino acid residues on its N-terminal region that are not present on the skeletal form, making this protein a promising analyte for indicating cardiac specificity. cTnI follows similar release pattern as CK-MB within the first 15 hours of mydegree Cardial infarction (MI), but remains elevated in serum up to 6 days. This observation and the unique amino sequence of cTnI make it an important marker of MI.
TnI is suitable for use as a standard in immunoassay for early detection of MI, immunogen for antiserum production and tracer for iodination.
Source Note
Human heart tissue. Blood sample from the tissue donors was tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies, HCV, and syphilis.
Analysis
Purity > 98 % (SDS-PAGE).
Immunological activity confirmed by reaction with monoclonal antibody that is specific for the troponin I cardiac isoform in immunoblotting.
TnI concentration was determined spectrophotometrically using A (0.1 %, 280 nm, 1 cm) equal to 0.42. This coefficient was calculated from the amino composition of human cTnI (FEBS Lett, 270, 57-61).
Immunological activity confirmed by reaction with monoclonal antibody that is specific for the troponin I cardiac isoform in immunoblotting.
TnI concentration was determined spectrophotometrically using A (0.1 %, 280 nm, 1 cm) equal to 0.42. This coefficient was calculated from the amino composition of human cTnI (FEBS Lett, 270, 57-61).
Preparation and Storage
At -20 degree C (-70 degree C for long term storage)
References
Search Troponin I references from PubMed.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
troponin I cardiac, partial
Similar Products
Product Notes
The cTnI (Catalog #AAA664049) is a Protein and is intended for research purposes only. The product is available for immediate purchase. TnI is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium sensitivity to striated muscle actomyosin ATPase activity. TnI exists in three isoforms, one for slow-twitch skeletal muscle, one for fast twitch skeletal muscle and one for cardiac muscle. The amino acid sequences of two skeletal (skTnI) and one cTnI forms how 40% dissimilarity. Furthermore, the human cTnI has additional amino acid residues on its N-terminal region that are not present on the skeletal form, making this protein a promising analyte for indicating cardiac specificity. cTnI follows similar release pattern as CK-MB within the first 15 hours of mydegree Cardial infarction (MI), but remains elevated in serum up to 6 days. This observation and the unique amino sequence of cTnI make it an important marker of MI. TnI is suitable for use as a standard in immunoassay for early detection of MI, immunogen for antiserum production and tracer for iodination. Researchers should empirically determine the suitability of the cTnI for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Troponin I Cardiac, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
