Mouse Connective tissue growth factor ELISA Kit | Ctgf elisa kit

Mouse Connective tissue growth factor ELISA Kit

Cat. Num. AAA763358

• Gene Names: CTGF; CCN2; NOV2; HCS24; IGFBP8
• Reactivity: Mouse
• Synonyms: Connective tissue growth factor; Mouse Connective tissue growth factor ELISA Kit; Ctgf/IGFBP8/CCN2/HCS24/IGFBP-8/IBP-8/NOV2/Hypertrophic chondrocyte-specific protein 24/IGF-binding protein 8/CCN family member 2/Insulin-like growth factor-binding protein 8; Ctgf elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of CTGF. No significant cross-reactivity or interference between CTGF and analogues was observed.
• Sequence Length: 349

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 62.5-4000pg/ml
• Sensitivity: <37.5pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CTGF were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CTGF were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Ctgf elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CTGF antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CTGF antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CTGF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CTGF can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 180924
• NCBI GeneID: 1490
• Molecular Weight: 35,205 Da
• NCBI Official Full Name: connective tissue growth factor
• NCBI Official Synonym Full Names: connective tissue growth factor
• NCBI Official Symbol: CTGF
• NCBI Official Synonym Symbols: CCN2; NOV2; HCS24; IGFBP8
• NCBI Protein Information: connective tissue growth factor
• UniProt Protein Name: Connective tissue growth factor
• Protein Family: Connective tissue growth factor
• UniProt Gene Name: CTGF
• UniProt Synonym Gene Names: CCN2; HCS24; IGFBP8; IBP-8; IGF-binding protein 8; IGFBP-8
• UniProt Entry Name: CTGF_HUMAN

NCBI Description

The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]

Uniprot Description

CTGF: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Belongs to the CCN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cell adhesion; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6q23.1

Cellular Component: cell cortex; cis-Golgi network; cytosol; extracellular region; extracellular space; Golgi apparatus; intracellular membrane-bound organelle; perinuclear region of cytoplasm; plasma membrane; proteinaceous extracellular matrix

Molecular Function: fibronectin binding; growth factor activity; heparin binding; insulin-like growth factor binding; integrin binding; protein binding; protein C-terminus binding

Biological Process: angiogenesis; cartilage condensation; cell adhesion; cell differentiation; cell migration; cell-cell signaling; cell-matrix adhesion; cellular lipid metabolic process; epidermis development; fibroblast growth factor receptor signaling pathway; gene expression; integrin-mediated signaling pathway; lung development; organ senescence; ossification; positive regulation of caspase activity; positive regulation of cell activation; positive regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; positive regulation of stress fiber formation; regulation of cell growth; regulation of chondrocyte differentiation; response to amino acid stimulus; response to anoxia; response to estradiol stimulus; response to glucose stimulus; response to mineralocorticoid stimulus; response to peptide hormone stimulus; response to wounding; signal transduction; tissue homeostasis; transcription initiation from RNA polymerase II promoter