CTCFL / Transcriptional Repressor CTCFL Recombinant Protein | CTCFL recombinant protein

Human CTCFL / Transcriptional Repressor CTCFL Recombinant Protein

• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: CTCFL / Transcriptional Repressor CTCFL; Human CTCFL / Transcriptional Repressor CTCFL Recombinant Protein; Transcriptional repressor CTCFL; Brother of the regulator of imprinted sites; CCCTC-binding factor; CTCF paralog; CTCF-like protein; Cancer/testis antigen 27; CT27; Zinc finger protein CTCF-T; BORIS; CTCFL recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 663

• Applicable Applications for CTCFL recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Interacts with histones, PRMT7 and SETD1A. Interacts (via N-terminus) with BAG6/BAT3.
• Entry Function: Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity). Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 313104098
• UniProt Accession #: Q8NI51
• NCBI Official Full Name: Transcriptional repressor CTCFL
• UniProt Protein Name: Transcriptional repressor CTCFL
• Protein Family: Transcriptional repressor
• UniProt Gene Name: CTCFL
• UniProt Synonym Gene Names: BORIS; CT27
• UniProt Entry Name: CTCFL_HUMAN

Uniprot Description

BORIS: Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites. Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters. Interacts with histones, PRMT7 and SETD1A. Interacts (via N-terminus) with BAG6/BAT3. Testis specific. Specifically expressed in primary spermatocytes. Belongs to the CTCF zinc-finger protein family.

Protein type: DNA-binding; Cancer Testis Antigen (CTA); C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 20q13.31

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; histone binding; sequence-specific DNA binding; metal ion binding

Biological Process: histone methylation; genetic imprinting; transcription, DNA-dependent; DNA methylation during gametogenesis; positive regulation of transcription, DNA-dependent; regulation of histone H3-K4 methylation; positive regulation of transcription from RNA polymerase II promoter; cell cycle