Cystatin 4 (CST4) Recombinant Protein | CST4 recombinant protein

Recombinant Cystatin 4 (CST4)

Cat. Num. AAA2009929

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cystatin 4 (CST4); Recombinant Cystatin 4 (CST4); CST4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-SSSKEENRII PGGIYDADLN DEWVQRALHF AISEYNKATE DEYYRRPLQV LRAREQTFGG VNYFFDVEVG RTICTKSQPN LDTCAFHEQP ELQKKQLCSF EIYEVPWEDR MSLVNSRCQE A
• Sequence Length: 141

• Applicable Applications for CST4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ser21~Ala141 (Accession # P01036) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4503109
• NCBI GeneID: 1472
• NCBI Accession #: NP_001890.1
• NCBI GenBank Nucleotide #: NM_001899.2
• UniProt Accession #: P01036; Q9UBI5; Q9UCS9
• Molecular Weight: 15.4kDa
• NCBI Official Full Name: cystatin-S
• NCBI Official Synonym Full Names: cystatin S
• NCBI Official Symbol: CST4
• NCBI Protein Information: cystatin-S; cystatin 4; cystatin-4; cystatin-SA-III; salivary acidic protein 1
• UniProt Protein Name: Cystatin-S
• Protein Family: Cystatin
• UniProt Gene Name: CST4
• UniProt Entry Name: CYTS_HUMAN

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a type 2 salivary cysteine peptidase inhibitor. The protein is an S-type cystatin, based on its high level of expression in saliva, tears and seminal plasma. The specific role in these fluids is unclear but antibacterial and antiviral activity is present, consistent with a protective function. [provided by RefSeq, Jul 2008]

Uniprot Description

CST4: This protein strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively. Belongs to the cystatin family.

Protein type: Secreted, signal peptide; Secreted; Inhibitor

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: extracellular space

Molecular Function: cysteine protease inhibitor activity

Biological Process: negative regulation of proteolysis; retinal homeostasis; detection of chemical stimulus involved in sensory perception of bitter taste