Protein kinase Casein Kinase 2 alpha (PKCK2) Recombinant Protein | PKCK2 recombinant protein

Protein kinase Casein Kinase 2 alpha (PKCK2), 1-391aa, Human, His tag, E Coli

Cat. Num. AAA203045

• Gene Names: CSNK2A1; CKII; Cka1; Cka2; CK2A1; OCNDS; CSNK2A3
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: Protein kinase Casein Kinase 2 alpha (PKCK2); 1-391aa; Human; His tag; E Coli; Casein kinase II alpha 1 subunit isoform a; CSNK2A1; CK2A1; Protein kinase Casein Kinase 2 alpha; PKCK2; CK II; EC 2.7.11.1; Casein kinase II subunit alpha; Casein kinase II alpha 1 subunit isoform a Casein kinase 2 alpha 1 polypeptide; Casein kinase II alpha 1 subunit; Casein kinase II alpha subunit; CK2 alpha; CK2 catalytic subunit alpha; CKII.; PKCK2 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris pH8.0, 500mM NaCl, 1mM DTT, 50% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSGPVPSRAR VYTDVNTHRP REYWDYESHV VEWGNQDDYQ LVRKLGRGKY SEVFEAINIT NNEKVVVKIL KPVKKKKIKR EIKILENLRG GPNIITLADI VKDPVSRTPA LVFEHVNNTD FKQLYQTLTD YDIRFYMYEI LKALDYCHSM GIMHRDVKPH NVMIDHEHRK LRLIDWGLAE FYHPGQEYNV RVASRYFKGP ELLVDYQMYD YSLDMWSLGC MLASMIFRKE PFFHGHDNYD QLVRIAKVLG TEDLYDYIDK YNIELDPRFN DILGRHSRKR WERFVHSENQ HLVSPEALDF LDKLLRYDHQ SRLTAREAME HPYFYTVVKD QARMGSSSMP GGSTPVSSAN MMSGISSVPT PSPLGPLAGS PVIAAANPLG MPVPAAAGAQ Q
• Sequence Length: 391

• Applicable Applications for PKCK2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PKCK2 recombinant protein

Protein Kinase Casein Kinase 2 (PKCK2, also called CK2) is a ubiquitous Ser/Thr kinase expressed in all eukaryotes. PKCK2 is a tetramer composed of two catalytic kinase domains, alpha subunits, and two identical regulatory beta subunits. It has been implicated in cell cycle control, DNA repair, regulation of the circadian rhythm, and other cellular processes. Recombinant human PKCK2, fused to His tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PKCK2 recombinant protein

Signal Transduction

References

Kim YK., et al. (2006) J Biol Chem. 281 (48):36752-7.

NCBI and Uniprot Product Information

• NCBI GI #: 4503095
• NCBI GeneID: 1457
• NCBI Accession #: NP_001886
• NCBI GenBank Nucleotide #: NM_001895.3
• UniProt Accession #: P68400; P19138; P20426; Q14013; Q5U065; B4DYS6; D3DVV8; Q8NEV1
• Molecular Weight: 47.3 kDa (411 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: casein kinase II subunit alpha isoform a
• NCBI Official Synonym Full Names: casein kinase 2 alpha 1
• NCBI Official Symbol: CSNK2A1
• NCBI Official Synonym Symbols: CKII; Cka1; Cka2; CK2A1; OCNDS; CSNK2A3
• NCBI Protein Information: casein kinase II subunit alpha
• UniProt Protein Name: Casein kinase II subunit alpha
• UniProt Gene Name: CSNK2A1
• UniProt Synonym Gene Names: CK2A1; CK II alpha

NCBI Description

Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. [provided by RefSeq, Jul 2014]

Uniprot Description

Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV. Phosphorylates PML at 'Ser-565' and primes it for ubiquitin-mediated degradation. Plays an important role in the circadian clock function by phosphorylating ARNTL/BMAL1 at 'Ser-90' which is pivotal for its interaction with CLOCK and which controls CLOCK nuclear entry (PubMed:11239457, PubMed:11704824, PubMed:16193064, PubMed:19188443, PubMed:20625391, PubMed:22406621). Phosphorylates CCAR2 at 'Thr-454' in gastric carcinoma tissue (PubMed:24962073).