CD114/CSF3R recombinant protein

CD114/CSF3R Recombinant Protein

Cat. Num. AAA3003834

• Gene Names: CSF3R; CD114; GCSFR
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is >85% (by SDS-PAGE).
• Synonyms: CD114/CSF3R; CD114/CSF3R Recombinant Protein; CD114/CSF3R recombinant protein

• Host: E Coli
• Purity/Purification: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is >85% (by SDS-PAGE).
• Form/Format: PBS, 4M Urea, PH7.4
• Concentration: >=0.5mg/ml (varies by lot)
• Sequence: ECGHISVSAPIVHLGDPITASCIIKQNCSHLDPEPQILWRLGAELQPGGRQQRLSDGTQESIITLPHLNHTQAFLSCCLNWGNSLQILDQVELRAGYPPAIPHNLSCLMNLTTSSLICQWEPGPETHLPTSFTLKSFKSRGNCQTQGDSILDCVPKDGQSHCCIPRKHLLLYQNMGIWVQAENALGTSMSPQLCLDPMDVVKLEPPMLRTMDPSPEAAPPQAGCLQLCWEPWQPGLHINQKCELRHKPQRGEASWALVGPLPLEALQYELCGLLPATAYTLQIRCIRWPLPGHWSDWSPSLELRTTERAPTVRLDTWWRQRQLDPRTVQLFWKPVPLEEDSGRIQGYVVSWRPSGQAGAILPLCNTTELSCTFHLPSEAQEVALVAYNSAGTSRPTPVVFSESRGPALTRLHAMARDPHSLWVGWEPPNPWPQGYVIEWGLGPPSASNSNKTWRMEQNGRATGFLLKENIRPFQLYEIIVTPLYQDTMGPSQHVYAYSQEMAPSHAPELHLKHIGKTWAQLEWVPEPPELGKSPLTHYTIFWTNAQNQSFSAILNASSRGFVLHGLEPASLYHIHLMAASQAGATNSTVLTLMTLTPEGSELH

• Tag: His-tag in C-terminal
• Expression Vector: pet-22b(+)
• BP: 1821
• Restriction Site: NdeI-XhoI
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Testing Data

()

Related Product Information for CD114/CSF3R recombinant protein

Background: The diverse biological activities of G-CSF are initiated by the binding of G-CSF to a specific receptor (G-CSFR) that belongs to the cytokine/hematopoietic receptor superfamily. In contrast to the majority of hematopoietic receptors that are activated through the formation of heteromeric complexes composed of alpha, beta and sometimes gamma subunits, G-CSFR proteins are believed to form homodimeric complexes upon ligand binding. Four distinct alternative splice variants of G-CSFR have been described, one of which exists as a soluble receptor protein. Although G-CSFR lacks consensus motifs in its cytoplasmic domains that are characteristic of kinase activities, certain sequences have been identified that are conserved among several members of the cytokine receptor superfamily. For example, the carboxy terminal regions of G-CSFR contain a domain, designated box 3, that is only shared with the IL-6R subunit, gp130.

NCBI and Uniprot Product Information

• NCBI GI #: 27437045
• NCBI GeneID: 1441
• NCBI Accession #: NP_758519.1
• NCBI GenBank Nucleotide #: NM_172313.2
• UniProt Accession #: Q99062
• Molecular Weight: 92,156 Da
• NCBI Official Full Name: granulocyte colony-stimulating factor receptor isoform d
• NCBI Official Synonym Full Names: colony stimulating factor 3 receptor (granulocyte)
• NCBI Official Symbol: CSF3R
• NCBI Official Synonym Symbols: CD114; GCSFR
• NCBI Protein Information: granulocyte colony-stimulating factor receptor; G-CSF-R; CD114 antigen; G-CSF receptor
• UniProt Protein Name: Granulocyte colony-stimulating factor receptor
• UniProt Gene Name: CSF3R
• UniProt Synonym Gene Names: GCSFR; G-CSF receptor; G-CSF-R
• UniProt Entry Name: CSF3R_HUMAN

NCBI Description

The protein encoded by this gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Alternatively spliced transcript variants have been described. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. [provided by RefSeq, Aug 2010]

Uniprot Description

GCSFR: the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variant isoforms have been described.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p35-p34.3

Cellular Component: integral to plasma membrane; extracellular region

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; receptor activity

Biological Process: neutrophil chemotaxis; cytokine and chemokine mediated signaling pathway; defense response; signal transduction; cell adhesion

Disease: Neutrophilia, Hereditary