Macrophage Colony stimulating Factor Recombinant Protein | M-CSF recombinant protein

Human Macrophage Colony stimulating Factor Recombinant

Cat. Num. AAA553155

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Macrophage Colony stimulating Factor; Human Macrophage Colony stimulating Factor Recombinant; CSF-1; csf1; m-csf; macrophage colony stimulting; mcsf; M-CSF recombinant protein

• Host: Baculovirus infected Bombyx Mori
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant M-CSF was lyophilized from a 0.2 um filtered PBS solution pH 7.0 containing 1% HSA, 3% mannitol.
• Sequence: MTAPGAAGRC PPTTWLGSLL LLVCLLASRS ITEEVSEYCS HMIGSGHLQS LQRLIDSQME TSCQITFEFV DQEQLKDPVC YLKKAFLLVQ DIMEDTMRFR DNTPNAIAIV QLQELSLRLK SCFTKDYEEH DKACVRTFYE TPLQLLEKVK NVFNETKNLL DKDWNIFSKN CNNSFAECSS QDVVTKPDCN CLYPKAIPSS DPASVSPHQP LAPSMAPVAG LTWEDSEGTE GSSLLPGEQP LHTVDPGSAK QRPPRSTCQS FEPPETPVVK DSTIGGSPQP RPSVGAFNPG MEDILDSAMG TNWVPEEASG EASEIPVPQG TELSPSRPGG GSMQTEPARP SNFLSASSPL PASAKGQQPA DVTGTALPRV GPVRPTGQDW NHTPQKTDHP SALLRDPPEP GSPRISSLRP QGLSNPSTLS AQPQLSRSHS SGSVLPLGEL EGRRSTRDRR SPAEPEGGPA SEGAARPLPR FNSVPLTDTG HERQSEGSSS PQLQESVFHL LVPSVILVLL AVGGLLFYRW RRRSHQEPQR ADSPLEQPEG SPLTQDDRQV ELPV
• Sequence Length: 554

• Domain: four helical cytokine core
• Host Note: Optimized DNA sequence encoding Human M-CSF extracellular domain was expressed in baculovirus infected Bombyx Mori.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Human MCSF, generated by the proteolytic removal of the signal peptide and propeptide, The molecule has a calculated molecular mass of approximately 37 kDa.; ; Recombinant Human M-CSF is a disulfide-linked homodimeric protein consisting of two 149 amino acid residue subunits, and migrates as an approximately 42 kDa protein under non-reducing and as 20-21kDa under reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for M-CSF recombinant protein

M-CSF is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. After cell activation, B-cells and T- cells and also a number of tumor cell lines are capable also of synthesizing this factor. M-CSF has been found to be synthesized by uterine epithelial cells in vivo. The M-CSF receptor is identical with the proto- oncogene fms. It has been renamed CD115. The receptor is a transmembrane protein with an extracellular ligand- binding domain of 512 amino acids, an intramembrane domain of 25 amino acids, and a cytoplasmic domain of 435 amino acids encoding a tyrosine kinase.Human M-CSF is active in mouse and rat cells. The murine factor is active in rat cells but inactive in human cells.M- CSF is a specific factor in that the proliferation inducing activity is more or less restricted to the macrophage lineage. M-CSF is a potent stimulator of functional activities of monocytes.

Product Categories/Family for M-CSF recombinant protein

Cytokines

References

• gM-CSF and il-4 induce dendritic cell differentiation and disrupt osteoclastogenesis through M-CSF receptor shedding by up-regulation of tnf- converting enzyme (tace) .Blood, Sep 2009; 10.1182/blood-2009-04-215020.
• gM-CSF- and M-CSF-dependent macrophage phenotypes display differential dependence on type i interferon signaling .J. Leukoc. Biol., Aug 2009; 86: 411 - 421.
• M-CSF inhibition selectively targets pathological angiogenesis and lymphangiogenesis .J. Exp. Med., May 2009; 206: 1089 - 1102.
• c-reactive protein induces M-CSF release and macrophage proliferation .J. Leukoc. Biol., Feb 2009; 85: 262 - 267.
• M-CSF preferentially induces c-fos via erk to specify monopoiesis whereas g-csf directs granulopoiesis via shp2 .Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 2887.