Human crystallin, mu ELISA Kit | CRYM elisa kit

Human Mu-crystallin homolog, CRYM ELISA Kit

Cat. Num. AAA9319285

• Gene Names: CRYM; THBP; DFNA40
• Reactivity: Human
• Synonyms: crystallin; mu; Human Mu-crystallin homolog; CRYM ELISA Kit; Human Mu-crystallin homolog (CRYM) ELISA kit; DFNA40; THBP; NADP-regulated thyroid-hormone binding protein; OTTHUMP00000115878; CRYM elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human CRYM and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.25 ng/ml - 8 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated CRYM concentrations in Human serum, plasma and other body fluids. Using Purified Human CRYM antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CRYM and CRYM antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CRYM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for CRYM elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated CRYM concentrations in Human serum, plasma and other body fluids. Using Purified Human CRYM antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CRYM and CRYM antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CRYM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 62241008
• NCBI GeneID: 1428
• NCBI Accession #: NP_001014444.1
• NCBI GenBank Nucleotide #: NM_001014444.2
• UniProt Accession #: Q14894; Q5HYB7; D5MNX0
• Molecular Weight: 33,776 Da
• NCBI Official Full Name: ketimine reductase mu-crystallin isoform 2
• NCBI Official Synonym Full Names: crystallin, mu
• NCBI Official Symbol: CRYM
• NCBI Official Synonym Symbols: THBP; DFNA40
• NCBI Protein Information: ketimine reductase mu-crystallin; mu-crystallin homolog; thiomorpholine-carboxylate dehydrogenase; NADP-regulated thyroid-hormone binding protein
• UniProt Protein Name: Ketimine reductase mu-crystallin
• Protein Family: Ketimine reductase
• UniProt Gene Name: CRYM
• UniProt Synonym Gene Names: THBP
• UniProt Entry Name: CRYM_HUMAN

NCBI Description

Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

CRYM: Specifically catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). Binds thyroid hormone which is a strong reversible inhibitor. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors. Belongs to the ornithine cyclodeaminase family.

Protein type: Lyase; EC 1.5.1.25

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: mitochondrion; cytoplasm; nucleus

Molecular Function: thiomorpholine-carboxylate dehydrogenase activity; protein homodimerization activity; NADP binding; transcription corepressor activity

Biological Process: sensory perception of sound; negative regulation of transcription from RNA polymerase II promoter; thyroid hormone metabolic process

Disease: Deafness, Autosomal Dominant 40