C-reactive/CRP Recombinant Protein | CRP recombinant protein

Human CellExp C-reactive / CRP, human recombinant

Cat. Num. AAA845195

• Gene Names: CRP; PTX1
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: C-reactive/CRP; Human CellExp C-reactive / CRP; human recombinant; CRP; PTX1; C-reactive protein; CRP recombinant protein

• Host: HEK 293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.; Appearance: Lyophilized powder
• Sequence Length: 224

• Applicable Applications for CRP recombinant protein: SDS-PAGE; HPLC
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 200 ug/ml. Solubilize for 30 to 60 min. at RT with occasional gentle mixing. Do not vortex.
• Endotoxin Level: < 0.1 ng/mug of protein (<1EU/mug).
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for CRP recombinant protein

Background: C-reactive protein (CRP) is a member of the pentraxin family of proteins that are characterized by a cyclic pentameric structure. Human CRP gene encodes a 224 amino acids precursor. The mature human CRP protein has 206 amino acids that are noncovalently linked to form the pentameter. Human CRP shares 71% and 64% amino acid sequence homology with mouse and rat respectively. CRP, synthesized by hepatocytes, is a major acute phase serum protein in human. IL6, IL1 and glucocorticoids are the major inducer of the CRP gene. The physiological role of CRP is to bind to phosphocholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system. CRP binds to phosphocholine on microbes and damaged cells and enhances phagocytosis by macrophages. Thus, CRP participates in the clearance of necrotic and apoptotic cells. CRP rises up to 50,000-fold in acute inflammation, such as infection. It rises above normal limits within 6 hours, and peaks at 48 hours. Its half-life is constant, and therefore its level is mainly determined by the rate of production. It has been shown that high levels of CRP in humans is associated with an increased risk of cardiovascular diseases.

Product Categories/Family for CRP recombinant protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteinsProteins and Enzymes; Human cell expressed recombinant proteins Proteins and Enzymes; Proteins and Enzymes (A-Z) Cytokines; Growth Factors & Hormones; Cytokines & Growth Factor

NCBI and Uniprot Product Information

• NCBI GI #: 55770842
• NCBI GeneID: 1401
• NCBI Accession #: NP_000558.2
• NCBI GenBank Nucleotide #: NM_000567.2
• UniProt Accession #: P02741; Q08AK3; Q8WW75; A8K078; D3DVD9; D3DVE0
• Molecular Weight: This is a 206 amino acids protein with polyhistidine tag at C-terminus, and has a calculated MW of 24 kDa. The predicted N-terminal is Phe17. DTT-reduced protein migrates as 26 kDa protein.
• NCBI Official Full Name: C-reactive protein
• NCBI Official Synonym Full Names: C-reactive protein, pentraxin-related
• NCBI Official Symbol: CRP
• NCBI Official Synonym Symbols: PTX1
• NCBI Protein Information: C-reactive protein
• UniProt Protein Name: C-reactive protein
• Protein Family: Cryparin
• UniProt Gene Name: CRP
• UniProt Synonym Gene Names: PTX1
• UniProt Entry Name: CRP_HUMAN

NCBI Description

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq, Sep 2009]

Uniprot Description

CRP: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Belongs to the pentaxin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q23.2

Cellular Component: extracellular space; growth cone; extracellular region; filopodium

Molecular Function: choline binding; protein binding; protein homodimerization activity; low-density lipoprotein receptor binding; complement component C1q binding; cholesterol binding; low-density lipoprotein binding; virion binding; calcium ion binding

Biological Process: protein polymerization; defense response to Gram-positive bacterium; response to ethanol; wound healing; negative regulation of vasodilation; positive regulation of superoxide release; response to lead ion; acute-phase response; response to hypoxia; opsonization; inflammatory response; complement activation, classical pathway; aging