CRISP3 recombinant protein

Recombinant Human CRISP3 Protein

Cat. Num. AAA8248835

• Gene Names: CRISP3; Aeg2; CRS3; SGP28; CRISP-3; dJ442L6.3
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: CRISP3; Recombinant Human CRISP3 Protein; Cysteine-rich secretory protein 3; CRISP-3; Specific granule protein of 28 kDa; SGP28; CRISP3 recombinant protein

• Host: Human cells
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 uM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Sequence: NEDKDPAFTA LLTTQTQVQR EIVNKHNELR RAVSPPARNM LKMEWNKEAA ANAQKWANQC NYRHSNPKDR MTSLKCGENL YMSSASSSWS QAIQSWFDEY NDFDFGVGPK TPNAVVGHYT QVVWYSSYLV GCGNAYCPNQ KVLKYYYVCQ YCPAGNWANR LYVPYEQGAP CASCPDNCDD GLCTNGCKYE DLYSNCKSLK LTLTCKHQLV RDSCKASCNC SNSIYVDHHH HHH
• Sequence Length: 268

• Applicable Applications for CRISP3 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Quality Control: Endotoxin :Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. ; ; Reconstituted protein solution can be stored at 2-8°C for 2-7 days. ; ; Aliquots of reconstituted samples are stable at -20°C for 3 months.

Related Product Information for CRISP3 recombinant protein

Recombinant Human CRISP3 Protein is produced by our mammalian expression system and the target gene encoding Asn21-Tyr245 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 300244562
• NCBI GeneID: 10321
• NCBI Accession #: NP_001177915.1
• NCBI GenBank Nucleotide #: NM_001190986.2
• UniProt Accession #: P54108; Q15512; Q3MJ82; Q53FA9; Q5JW83; Q9H108; A8K9S1; B2R8I8
• NCBI Official Full Name: cysteine-rich secretory protein 3 isoform 2
• NCBI Official Synonym Full Names: cysteine rich secretory protein 3
• NCBI Official Symbol: CRISP3
• NCBI Official Synonym Symbols: Aeg2; CRS3; SGP28; CRISP-3; dJ442L6.3
• NCBI Protein Information: cysteine-rich secretory protein 3
• UniProt Protein Name: Cysteine-rich secretory protein 3
• Protein Family: Cysteine-rich secretory protein
• UniProt Gene Name: CRISP3
• UniProt Synonym Gene Names: CRISP-3; SGP28
• UniProt Entry Name: CRIS3_HUMAN

NCBI Description

This gene encodes a member of the cysteine-rich secretory protein (CRISP) family within the CRISP, antigen 5 and pathogenesis-related 1 proteins superfamily. The encoded protein has an N-terminal CRISP, antigen 5 and pathogenesis-related 1 proteins domain, a hinge region, and a C-terminal ion channel regulator domain. This protein contains cysteine residues, located in both the N- and C-terminal domains, that form eight disulfide bonds, a distinguishing characteristic of this family. This gene is expressed in the male reproductive tract where it plays a role in sperm function and fertilization, and the female reproductive tract where it plays a role in endometrial receptivity for embryo implantation. This gene is upregulated in certain types of prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]

Uniprot Description

CRISP3: Belongs to the CRISP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p12.3

Cellular Component: extracellular region; extracellular space; specific granule