Cyclic AMP-responsive element-binding protein 1 Recombinant Protein | CREB1 recombinant protein

Recombinant Human Cyclic AMP-responsive element-binding protein 1

Cat. Num. AAA965545

• Gene Names: CREB1; CREB
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Cyclic AMP-responsive element-binding protein 1; Recombinant Human Cyclic AMP-responsive element-binding protein 1; CREB1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-341aa; Full Length
• Sequence: MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD
• Sequence Length: 341

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for CREB1 recombinant protein

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

Product Categories/Family for CREB1 recombinant protein

Immunology

References

Two distinct forms of active transcription factor CREB (cAMP response element binding protein) .Berkowitz L.A., Gilman M.Z.Proc. Natl. Acad. Sci. U.S.A. 87:5258-5262(1990) Multiple cDNA clones encoding nuclear proteins that bind to the tax-dependent enhancer of HTLV-1 all contain a leucine zipper structure and basic amino acid domain.Yoshimura T., Fujisawa J., Yoshida M.EMBO J. 9:2537-2542(1990) Diversification of cyclic AMP-responsive enhancer binding proteins-generated by alternative exon splicing.Waeber G., Meyer T.E., Hoeffler J.P., Habener J.F.Trans. Assoc. Am. Physicians 103:28-37(1990) Cyclic AMP-responsive DNA-binding protein structure based on a cloned placental cDNA.Hoeffler J.P., Meyer T.E., Yun Y., Jameson J.L., Habener J.F.Science 242:1430-1433(1988) Nucleotide and derived amino-acid sequences of the CRE-binding proteins from rat C6 glioma and HeLa cells.Short M.L., Manohar C.F., Furtado M.R., Ghadge G.D., Wolinsky S.M., Thimmapaya B., Jungmann R.A.Nucleic Acids Res. 19:4290-4290(1991) Cloning and expression of a novel CREB mRNA splice variant in human testis.Huang X., Zhang J., Lu L., Yin L., Xu M., Wang Y., Zhou Z., Sha J.Reproduction 128:775-782(2004) The promoter of the gene encoding 3',5'-cyclic adenosine monophosphate (cAMP) response element binding protein contains cAMP response elements evidence for positive autoregulation of gene transcription.Meyer T.E., Waeber G., Lin J., Beckmann W., Habener J.F.Endocrinology 132:770-780(1993) HBV X protein alters the DNA binding specificity of CREB and ATF-2 by protein-protein interactions.Maguire H.F., Hoeffler J.P., Siddiqui A.Science 252:842-844(1991) Human T-cell lymphotropic virus type I (HTLV-I) transcriptional activator, Tax, enhances CREB binding to HTLV-I 21-base-pair repeats by protein-protein interaction.Zhao L.J., Giam C.-Z.Proc. Natl. Acad. Sci. U.S.A. 89:7070-7074(1992) Calcium/calmodulin-dependent protein kinase types II and IV differentially regulate CREB-dependent gene expression.Matthews R.P., Guthrie C.R., Wailes L.M., Zhao X., Means A.R., McKnight G.S.Mol. Cell. Biol. 14:6107-6116(1994) Transcriptional activation of egr-1 by granulocyte-macrophage colony-stimulating factor but not interleukin 3 requires phosphorylation of cAMP response element-binding protein (CREB) on serine 133.Lee H.-J.J., Mignacca R.C., Sakamoto K.M.J. Biol. Chem. 270:15979-15983(1995) CREB is a regulatory target for the protein kinase Akt/PKB.Du K., Montminy M.J. Biol. Chem. 273:32377-32379(1998) Rsk-2 activity is necessary for epidermal growth factor-induced phosphorylation of CREB protein and transcription of c-fos gene.De Cesare D., Jacquot S., Hanauer A., Sassone-Corsi P.Proc. Natl. Acad. Sci. U.S.A. 95:12202-12207(1998) TORCs transducers of regulated CREB activity.Conkright M.D., Canettieri G., Screaton R., Guzman E., Miraglia L., Hogenesch J.B., Montminy M.Mol. Cell 12:413-423(2003) Small ubiquitin-related modifier-1 modification mediates resolution of CREB-dependent responses to hypoxia.Comerford K.M., Leonard M.O., Karhausen J., Carey R., Colgan S.P., Taylor C.T.Proc. Natl. Acad. Sci. U.S.A. 100:986-991(2003) Identification of a family of cAMP response element-binding protein coactivators by genome-scale functional analysis in mammalian cells.Iourgenko V., Zhang W., Mickanin C., Daly I., Jiang C., Hexham J.M., Orth A.P., Miraglia L., Meltzer J., Garza D., Chirn G.-W., McWhinnie E., Cohen D., Skelton J., Terry R., Yu Y., Bodian D., Buxton F.P., Zhu J., Song C., Labow M.A.Proc. Natl. Acad. Sci. U.S.A. 100:12147-12152(2003) The CREB coactivator TORC2 functions as a calcium- and cAMP-sensitive coincidence detector.Screaton R.A., Conkright M.D., Katoh Y., Best J.L., Canettieri G., Jeffries S., Guzman E., Niessen S., Yates J.R. III, Takemori H., Okamoto M., Montminy M.Cell 119:61-74(2004) A nuclear function of beta-arrestin1 in GPCR signaling regulation of histone acetylation and gene transcription.Kang J., Shi Y., Xiang B., Qu B., Su W., Zhu M., Zhang M., Bao G., Wang F., Zhang X., Yang R., Fan F., Chen X., Pei G., Ma L.Cell 123:833-847(2005) Serum/glucocorticoid-inducible kinase can phosphorylate the cyclic AMP response element binding protein, CREB.David S., Kalb R.G.FEBS Lett. 579:1534-1538(2005) PGC-1-related coactivator immediate early expression and characterization of a CREB/NRF-1 binding domain associated with cytochrome c promoter occupancy and respiratory growth.Vercauteren K., Pasko R.A., Gleyzer N., Marino V.M., Scarpulla R.C.Mol. Cell. Biol. 26:7409-7419(2006) EWSR1-CREB1 is the predominant gene fusion in angiomatoid fibrous histiocytoma.Antonescu C.R., Dal Cin P., Nafa K., Teot L.A., Surti U., Fletcher C.D., Ladanyi M.Genes Chromosomes Cancer 46:1051-1060(2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.Science 316:1160-1166(2007) A quantitative atlas of mitotic phosphorylation.Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.Sci. Signal. 2:RA46-RA46(2009) Regulation of genotoxic stress response by homeodomain-interacting protein kinase 2 through phosphorylation of cyclic AMP response element-binding protein at serine 271.Sakamoto K., Huang B.-W., Iwasaki K., Hailemariam K., Ninomiya-Tsuji J., Tsuji Y.Mol. Biol. Cell 21:2966-2974(2010) TOX3 is a neuronal survival factor that induces transcription depending on the presence of CITED1 or phosphorylated CREB in the transcriptionally active complex.Dittmer S., Kovacs Z., Yuan S.H., Siszler G., Kogl M., Summer H., Geerts A., Golz S., Shioda T., Methner A.J. Cell Sci. 124:252-260(2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.Sci. Signal. 4:RS3-RS3(2011) A p.D116G mutation in CREB1 leads to novel multiple malformation syndrome resembling CrebA knockout mouse.Kitazawa S., Kondo T., Mori K., Yokoyama N., Matsuo M., Kitazawa R.Hum. Mutat. 33:651-654(2012) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.J. Proteomics 96:253-262(2014)

NCBI and Uniprot Product Information

• NCBI GI #: 4758054
• NCBI GeneID: 1385
• NCBI Accession #: NP_004370.1
• NCBI GenBank Nucleotide #: NM_004379.3
• UniProt Accession #: P16220; P21934; Q6V963; Q9UMA7
• Molecular Weight: 40.7 kDa
• NCBI Official Full Name: cyclic AMP-responsive element-binding protein 1 isoform A
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 1
• NCBI Official Symbol: CREB1
• NCBI Official Synonym Symbols: CREB
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 1
• UniProt Protein Name: Cyclic AMP-responsive element-binding protein 1
• Protein Family: Cyclic AMP-responsive element-binding protein
• UniProt Gene Name: CREB1
• UniProt Synonym Gene Names: CREB-1; cAMP-responsive element-binding protein 1
• UniProt Entry Name: CREB1_HUMAN

NCBI Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CREB: a transcription factor of the leucine zipper family of DNA binding proteins. Binds as a homodimer to the cAMP-responsive element (CRE). Phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: axon; mitochondrial matrix; nucleoplasm; nucleus

Molecular Function: enzyme binding; histone acetyltransferase binding; Hsp70 protein binding; identical protein binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding; transcription cofactor activity; transcription factor activity

Biological Process: aging; axon guidance; circadian rhythm; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; lactation; memory; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; nerve growth factor receptor signaling pathway; Notch signaling pathway; phosphoinositide-mediated signaling; phospholipase C activation; pituitary gland development; positive regulation of apoptosis; positive regulation of fat cell differentiation; positive regulation of hormone secretion; positive regulation of lipid biosynthetic process; positive regulation of multicellular organism growth; positive regulation of osteoclast differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transcriptional preinitiation complex assembly; positive regulation of transforming growth factor-beta3 production; protein amino acid phosphorylation; protein stabilization; regulation of cell size; regulation of circadian rhythm; regulation of fibroblast proliferation; response to activity; response to glucagon stimulus; response to hypoxia; response to nicotine; response to organic substance; secretory granule organization and biogenesis; signal transduction; stress-activated MAPK cascade; synaptic transmission; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription from RNA polymerase II promoter; transforming growth factor beta receptor signaling pathway; viral reproduction; visual learning

Disease: Histiocytoma, Angiomatoid Fibrous