CASP2 And RIPK1 Domain Containing Adaptor With Death Domain Protein (CRADD) Recombinant Protein | CRADD recombinant protein

Recombinant CASP2 And RIPK1 Domain Containing Adaptor With Death Domain Protein (CRADD)

Cat. Num. AAA2011215

• Gene Names: CRADD; MRT34; RAIDD
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: CASP2 And RIPK1 Domain Containing Adaptor With Death Domain Protein (CRADD); Recombinant CASP2 And RIPK1 Domain Containing Adaptor With Death Domain Protein (CRADD); CRADD recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with a N-terminal His-tag, its sequence is listed below.; MGHHHHHHSGSEF-MEARDKQVLR SLRLELGAEV LVEGLVLQYL YQEGILTENH IQEINAQTTG LRKTMLLLDI LPSRGPKAFD TFLDSLQEFP WVREKLKKAR EEAMTDLPAG DRLTGIPSHI LNSSPSDRQI NQLAQRLGPE WEPMVLSLGL SQTDIYRCKA NHPHNVQSQV VEAFIRWRQR FGKQATFQSL HNGLRAVEVD PSLLLHMLE
• Sequence Length: 199

• Applicable Applications for CRADD recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Met1~Glu199 (Accession # P78560) with a N-terminal His-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 4503031
• NCBI GeneID: 8738
• NCBI Accession #: NP_003796.1
• NCBI GenBank Nucleotide #: NM_003805.3
• UniProt Accession #: P78560
• Molecular Weight: 24.2kDa
• NCBI Official Full Name: death domain-containing protein CRADD
• NCBI Official Synonym Full Names: CASP2 and RIPK1 domain containing adaptor with death domain
• NCBI Official Symbol: CRADD
• NCBI Official Synonym Symbols: MRT34; RAIDD
• NCBI Protein Information: death domain-containing protein CRADD; death adaptor molecule RAIDD; caspase and RIP adaptor with death domain; RIP-associated protein with a death domain; RIP-associated ICH1/CED3-homologous protein with death domain
• UniProt Protein Name: Death domain-containing protein CRADD
• Protein Family: Death domain-containing protein
• UniProt Gene Name: CRADD
• UniProt Synonym Gene Names: RAIDD
• UniProt Entry Name: CRADD_HUMAN

NCBI Description

The protein encoded by this gene is a death domain (CARD/DD)-containing protein and has been shown to induce cell apoptosis. Through its CARD domain, this protein interacts with, and thus recruits, caspase 2/ICH1 to the cell death signal transduction complex that includes tumor necrosis factor receptor 1 (TNFR1A), RIPK1/RIP kinase, and numbers of other CARD domain-containing proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

CRADD: Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex. Defects in CRADD are the cause of mental retardation autosomal recessive type 34 (MRT34). A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRT34 is a non- syndromic form. Affected individuals have mildly delayed development and significantly impaired cognitive function, precluding independent living and self-care. Speech is rudimentary, but articulate; autism is not present.

Protein type: Adaptor/scaffold; Apoptosis

Chromosomal Location of Human Ortholog: 12q21.33-q23.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding, bridging; protein binding; protease binding

Biological Process: caspase activation; induction of apoptosis via death domain receptors; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest

Disease: Mental Retardation, Autosomal Recessive 34