Human Cytosolic Phospholipase A2 ELISA Kit | CPLA2 elisa kit

Human Cytosolic Phospholipase A2 ELISA Kit

Cat. Num. AAA14859

• Gene Names: PLA2G4A; PLA2G4; cPLA2-alpha
• Reactivity: Human
• Synonyms: Cytosolic Phospholipase A2; Human Cytosolic Phospholipase A2 ELISA Kit; CPLA2 elisa kit

• Reactivity: Human

• Assay Type: Quantitative Sandwich
• Detection Range: 62.5pg/ml-2000pg/ml
• Sensitivity: 10pg/ml
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for CPLA2 elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used for determine the level of CPLA2 (hereafter termed "analyte") in undiluted original Human serum, plasma or tissue homogenates samples. For other sample types please contact tech support to determine compatibility with this assay. This kit is not suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 23943920
• NCBI GeneID: 5321
• NCBI Accession #: NP_077734.1
• NCBI GenBank Nucleotide #: NM_024420.2
• UniProt Accession #: P47712; Q29R80; B1AKG4
• Molecular Weight: 85,239 Da
• NCBI Official Full Name: cytosolic phospholipase A2
• NCBI Official Synonym Full Names: phospholipase A2, group IVA (cytosolic, calcium-dependent)
• NCBI Official Symbol: PLA2G4A
• NCBI Official Synonym Symbols: PLA2G4; cPLA2-alpha
• NCBI Protein Information: cytosolic phospholipase A2; cPLA2; lysophospholipase; phospholipase A2 group IVA; phosphatidylcholine 2-acylhydrolase; calcium-dependent phospholipid-binding protein
• UniProt Protein Name: Cytosolic phospholipase A2
• Protein Family: Cytosolic phospholipase
• UniProt Gene Name: PLA2G4A
• UniProt Synonym Gene Names: CPLA2; PLA2G4; cPLA2
• UniProt Entry Name: PA24A_HUMAN

NCBI Description

This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. [provided by RefSeq, Jul 2008]

Uniprot Description

cPLA2: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Arachidonic acid is a precursor for the eicosanoids that are involved in hemodynamic regulation, inflammatory responses, and other cellular processes. Promotes cerebellar long-term depression and motor learning. Translocates to the Golgi and endoplasmic reticulum in a calcium-dependent fashion. Translocation and activation of at the ER facilitates the process of ER stress. Regulates the biogenesis of lipid droplets, a process dependent on JNK and ceramide kinase. Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+. The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Inhibited when in trimolecular complex with ANXA2 and S100A10. Phosphorylation of S727 relieves this inhibitory interaction, thus activating PLA2G4A.

Protein type: Lipid Metabolism - ether lipid; Phospholipase; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - alpha-linolenic acid; EC 3.1.1.5; EC 3.1.1.4; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; cytoplasm; cytoplasmic membrane-bound vesicle; mitochondrial inner membrane; cytosol

Molecular Function: phospholipase A2 activity; calcium-dependent phospholipid binding; calcium-dependent phospholipase A2 activity; calcium ion binding; lysophospholipase activity

Biological Process: icosanoid biosynthetic process; platelet activation; platelet activating factor biosynthetic process; phospholipid catabolic process; phospholipid metabolic process; icosanoid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; arachidonic acid metabolic process; blood coagulation; arachidonic acid secretion; regulation of cell proliferation