RNA polymerase II C-terminal domain phosphatase-like 1 (CPL1) Recombinant Protein | CPL1 recombinant protein

Recombinant Arabidopsis thaliana RNA polymerase II C-terminal domain phosphatase-like 1 (CPL1) , partial

• Gene Names: CPL1; ATCPL1; ATCPL2; C-terminal domain phosphatase-like 1; C-TERMINAL DOMAIN PHOSPHATASE-LIKE 1; F17L22.130; F17L22_130; FIERY 2; FRY2; RCF2; regulators of C-REPEAT BINDING FACTOR (CBF) gene expression 2; SHI4; SHINY 4
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: RNA polymerase II C-terminal domain phosphatase-like 1 (CPL1); Recombinant Arabidopsis thaliana RNA polymerase II C-terminal domain phosphatase-like 1 (CPL1); partial; CPL1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 967

• Species: Arabidopsis thaliana (Mouse-ear cress)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 79481163
• NCBI GeneID: 828254
• NCBI Accession #: NP_193898.3
• NCBI GenBank Nucleotide #: NM_118287.5
• UniProt Accession #: Q5YDB6; Q0WNZ7; Q56WT8; Q9SVT0
• Molecular Weight: 108,421 Da
• NCBI Official Full Name: C-terminal domain phosphatase-like 1
• NCBI Official Symbol: CPL1
• NCBI Official Synonym Symbols: ATCPL1; ATCPL2; C-terminal domain phosphatase-like 1; C-TERMINAL DOMAIN PHOSPHATASE-LIKE 1; F17L22.130; F17L22_130; FIERY 2; FRY2; RCF2; regulators of C-REPEAT BINDING FACTOR (CBF) gene expression 2; SHI4; SHINY 4
• NCBI Protein Information: C-terminal domain phosphatase-like 1
• UniProt Protein Name: RNA polymerase II C-terminal domain phosphatase-like 1
• Protein Family: RNA polymerase II C-terminal domain phosphatase
• UniProt Gene Name: CPL1
• UniProt Synonym Gene Names: CTD phosphatase-like 1

NCBI Description

encodes a a novel transcriptional repressor harboring two double-stranded RNA-binding domains and a region homologous to the catalytic domain of RNA polymerase II C-terminal domain phosphatases found in yeast and in animals that regulate gene transcription. Protein exhibits innate phosphatase activity in vitro. Mutants exhibit hyperresponsiveness to ABA, cold, and NaCl.

Uniprot Description

Processively dephosphorylates 'Ser-5' but not 'Ser-2' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II. Together with CPL2, required for male gametes fertility. Multifunctional regulator that modulates plant growth, stress, and phytohormones responses. Negative regulator of stress gene transcription involved in abscisic acid (ABA) mediated and jasmonic acid (JA) mediated signaling pathways, NaCl, osmotic stress, wounding, and cold resistance. Regulates negatively the expression of jasmonic acid (JA) biosynthetic genes in response to wounding (PubMed:11874572, PubMed:12149434, PubMed:12149453, PubMed:15388846, PubMed:18506580, PubMed:18764923). Forms a complex with RCF3 that modulates co-transcriptional processes such as mRNA capping and polyadenylation, and functions to repress stress-inducible gene expression (PubMed:23874224). Dephosphorylates RCF3 (PubMed:26227967). Involved in the dephosphorylation of EIF4A3. This dephosphorylation retains EIF4A3 in the nucleus and limits its accumulation in the cytoplasm. Is essential for the degradation of the nonsense-mediated mRNA decay (NMD) transcripts (PubMed:26887918).