Cytoplasmic Polyadenylation Element Binding Protein 1 Recombinant Protein | CPEB1 recombinant protein

Recombinant Cytoplasmic Polyadenylation Element Binding Protein 1 (CPEB1)

Cat. Num. AAA2033505

• Gene Names: CPEB1; CPEB; CPEB-1; h-CPEB; CPE-BP1; hCPEB-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Cytoplasmic Polyadenylation Element Binding Protein 1; Recombinant Cytoplasmic Polyadenylation Element Binding Protein 1 (CPEB1); CEBP; CPE-BP1; CPEB; CPEB1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: CKVFLGGVPW DITEAGLVNT FRVFGSLSVE WPGKDGKHPR CPPKGYVYLV FELEKSVRSL LQACSHDPLS PDGLSEYYFK MSSRRMRCKE VQVIPWVLAD SNFVRSPSQR LDPSRTVFVG ALHGMLNAEA LAAILNDLFG GVVYAGIDTD KHKYPIGSGR VTFNNQRSYL KAVSAAFVEI KTTKFTKKVQ IDPYLEDSLC HICSSQPGPF FCRDQVCFKY FCRSCWHWRH SMEGLRHHSPLMRNQKNRDS S
• Sequence Length: 561

• Applicable Applications for CPEB1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 32.2kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Cys311~Ser561
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 29792282
• NCBI GeneID: 64506
• Molecular Weight: 53,635 Da
• NCBI Official Full Name: Cytoplasmic polyadenylation element binding protein 1
• NCBI Official Synonym Full Names: cytoplasmic polyadenylation element binding protein 1
• NCBI Official Symbol: CPEB1
• NCBI Official Synonym Symbols: CPEB; CPEB-1; h-CPEB; CPE-BP1; hCPEB-1
• NCBI Protein Information: cytoplasmic polyadenylation element-binding protein 1
• UniProt Protein Name: Cytoplasmic polyadenylation element-binding protein 1
• UniProt Gene Name: CPEB1
• UniProt Synonym Gene Names: CPEB; CPE-BP1; CPE-binding protein 1; h-CPEB; hCPEB-1
• UniProt Entry Name: CPEB1_HUMAN

NCBI Description

This gene encodes a member of the cytoplasmic polyadenylation element binding protein family. This highly conserved protein binds to a specific RNA sequence, called the cytoplasmic polyadenylation element, found in the 3' untranslated region of some mRNAs. The encoded protein functions in both the cytoplasm and the nucleus. It is involved in the regulation of mRNA translation, as well as processing of the 3' untranslated region, and may play a role in cell proliferation and tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

CPEB: cytoplasmic polyadenylation element binding protein 1. Binds to the protein CPE and drives its polyadenylation. Activated by the protein kinase AurA. Insulin and progesterone stimulate AurA-catalyzed phosphorylation of CPEB in oocytes. Neuronal CPEB regulates local protein synthesis and stabilizes synapse-specific long-term facilitation in aplysia.

Protein type: RNA-binding; Translation

Chromosomal Location of Human Ortholog: 15q25.2

Cellular Component: cell junction; cytoplasm; dendrite; neuron projection; nucleoplasm; nucleus; postsynaptic density; postsynaptic membrane; synapse

Molecular Function: metal ion binding; mRNA 3'-UTR binding; nucleotide binding; ribosome binding; translation factor activity, nucleic acid binding; translation repressor activity, nucleic acid binding

Biological Process: cellular response to insulin stimulus; mRNA processing; translation