Rat Ceruloplasmin ELISA Kit | Cp elisa kit

Rat Ceruloplasmin ELISA Kit

Cat. Num. AAA2881040

• Gene Names: Cp; CERP
• Reactivity: Rat
• Synonyms: Ceruloplasmin; Rat Ceruloplasmin ELISA Kit; Ferroxidase; Cp; 1.16.3.1; Cp elisa kit

• Reactivity: Rat
• Sequence Length: 1059

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Cp elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 2506226
• NCBI GeneID: 24268
• UniProt Accession #: P13635; Q64719
• Molecular Weight: 120,841 Da
• NCBI Official Full Name: Ceruloplasmin
• NCBI Official Synonym Full Names: ceruloplasmin (ferroxidase)
• NCBI Official Symbol: Cp
• NCBI Official Synonym Symbols: CERP
• NCBI Protein Information: ceruloplasmin
• UniProt Protein Name: Ceruloplasmin
• Protein Family: Ceruloplasmin
• UniProt Gene Name: Cp
• UniProt Entry Name: CERU_RAT

NCBI Description

copper-containing ferroxidase that promotes iron incorporation into transferrin; plays a role in iron metabolism and homeostatis [RGD, Feb 2006]

Uniprot Description

CP: Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense. Defects in CP are the cause of aceruloplasminemia (ACERULOP). It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances. Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2. Belongs to the multicopper oxidase family.

Protein type: Oxidoreductase; Secreted, signal peptide; EC 1.16.3.1; Secreted; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll

Cellular Component: anchored to plasma membrane; cell; extracellular space; lysosomal membrane

Molecular Function: chaperone binding; copper ion binding; ferroxidase activity

Biological Process: aging; cellular iron ion homeostasis; copper ion transport; liver development; lung development; plasma membrane copper ion transport; response to copper ion; response to nutrient