Typical Testing Data/Standard Curve (for reference only)
COVID 19 Nucleocapsid (NP) Coronavirus ELISA Kit | COVID-19 elisa kit
SARS-CoV-2 Nucleocapsid Protein ELISA Kit
Synonyms
COVID 19 Nucleocapsid (NP) Coronavirus; SARS-CoV-2 Nucleocapsid Protein ELISA Kit; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Nucleocapsid; Nucleoprotein (NP); COVID-19 elisa kit
Specificity
This kit recognizes SARS-CoV-2 N protein in the tested samples. No significant cross-reactivity or interference between N protein and analogues was observed.
Samples
Serum, plasma and other appropriate biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.39-25ng/mL
Sensitivity
0.12ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level SARS-CoV-2 N protein were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level SARS-CoV-2 N protein were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
The unopened kit can be stable for 6 months at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for COVID-19 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of SARS-CoV-2 Nucleocapsid protein (N protein) concentrations in serum, plasma and other appropriate biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SARS-CoV-2 N protein. Samples (or Standards) and biotinylated detection antibody specific for SARS-CoV-2 N protein are added to the micro ELISA plate wells. SARS-CoV-2 N protein would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SARS-CoV-2 N protein, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 +/- 2 nm. The OD value is proportional to the concentration of SARS- CoV-2 N protein. You can calculate the concentration of SARS-CoV-2 N protein in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SARS-CoV-2 N protein. Samples (or Standards) and biotinylated detection antibody specific for SARS-CoV-2 N protein are added to the micro ELISA plate wells. SARS-CoV-2 N protein would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SARS-CoV-2 N protein, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 +/- 2 nm. The OD value is proportional to the concentration of SARS- CoV-2 N protein. You can calculate the concentration of SARS-CoV-2 N protein in the samples by comparing the OD of the samples to the standard curve.
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Product Notes
The COVID-19 (Catalog #AAA2563929) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "COVID 19 Nucleocapsid (NP) Coronavirus, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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