Typical Testing Data/Standard Curve (for reference only)
COVID 19 high sensitivity Spike S1 IgG Coronavirus ELISA Kit | COVID-19 elisa kit
High-sensitive SARS-CoV-2 S1 IgG ELISA Kit
Synonyms
COVID 19 high sensitivity Spike S1 IgG Coronavirus; High-sensitive SARS-CoV-2 S1 IgG ELISA Kit; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike S1 IgG; COVID-19 elisa kit
Specificity
We have tested this assay in blood samples of 60 well-characterized COVID-19 patients, and 58 of 60 patients were anti-SARS-CoV-2 S1 IgG positive. 20 blood samples from healthy individuals were tested, and none of them were positive. The sensitivity of this assay is >= 96%, and the specificity of this assay is >=98%.
Samples
Serum, plasma or other specimen
Intra-assay Precision
Three different known levels of positive control were spiked into sample buffer as test samples. All samples were tested on the same plate to evaluate intra-assay precision of the kit. The Intra- assay precision of this kit is less than 5%.
Inter-assay Precision
Three different known levels of control were spiked into sample buffer as test samples. All samples were tested in 3 separate assays to evaluate intra-assay precision of the kit. Inter-assay precision of this kit is less than 7%.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for COVID-19 elisa kit
Intended Uses: SARS-CoV-2 S1 protein IgG ELISA Kit is a highly sensitive and specific immunoassay for the precision detection and quantitative measurement of IgG class antibodies against the spike S1 protein of SARS-CoV-2 virus in human blood samples in vitro.
Principle of the Assay: 96-well plates are coated with SARS-CoV-2 S1 protein that captures IgG antibodies against SARS- CoV-2 S1 protein in the sample. After washing away unbound materials, captured IgG against SARS-CoV-2 S1 protein is detected by anti-human IgG polyclonal antibodies conjugated with horse radish peroxidase (HRP). After washing step, the chromogenic substrate 3,3',5,5'- tetramethylbenzidine (TMB) is added. Color reaction is stopped by 2M H2SO4. The amount of anti- SARS- CoV-2 S1 antibodies captured inside the wells is proportional to the color density generated in the coupled oxidation-reduction reaction. The unknown sample concentration can be interpolated from a standard curve generated by Human anti-S1 monoclonal antibody (mAb).
Background/Introduction: SARS-CoV-2 is an enveloped virus with a positive-sense RNA genome and a nucleocapsid of helical symmetry. The SARS-CoV-2 entry into host cells is mediated by the transmembrane spike (S) glycoprotein, which is the main target of neutralizing antibodies upon infection. S comprises two functional subunits responsible for binding to the host cell receptor (S1 subunit) and fusion of the viral and cellular membranes (S2 subunit). The distal S1 subunit comprises the receptor- binding domain(s) and contributes to stabilization of the prefusion state of the membrane-anchored S2 subunit that contains the fusion
Principle of the Assay: 96-well plates are coated with SARS-CoV-2 S1 protein that captures IgG antibodies against SARS- CoV-2 S1 protein in the sample. After washing away unbound materials, captured IgG against SARS-CoV-2 S1 protein is detected by anti-human IgG polyclonal antibodies conjugated with horse radish peroxidase (HRP). After washing step, the chromogenic substrate 3,3',5,5'- tetramethylbenzidine (TMB) is added. Color reaction is stopped by 2M H2SO4. The amount of anti- SARS- CoV-2 S1 antibodies captured inside the wells is proportional to the color density generated in the coupled oxidation-reduction reaction. The unknown sample concentration can be interpolated from a standard curve generated by Human anti-S1 monoclonal antibody (mAb).
Background/Introduction: SARS-CoV-2 is an enveloped virus with a positive-sense RNA genome and a nucleocapsid of helical symmetry. The SARS-CoV-2 entry into host cells is mediated by the transmembrane spike (S) glycoprotein, which is the main target of neutralizing antibodies upon infection. S comprises two functional subunits responsible for binding to the host cell receptor (S1 subunit) and fusion of the viral and cellular membranes (S2 subunit). The distal S1 subunit comprises the receptor- binding domain(s) and contributes to stabilization of the prefusion state of the membrane-anchored S2 subunit that contains the fusion
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Product Notes
The COVID-19 (Catalog #AAA398013) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "COVID 19 high sensitivity Spike S1 IgG Coronavirus, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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