COVID 19 IgG Coronavirus Assay Kit | COVID-19 assay kit
recomLine SARS-CoV-2 IgG [Avidity]
Applications
Immunoassay (IA)
Synonyms
COVID 19 IgG Coronavirus; recomLine SARS-CoV-2 IgG [Avidity]; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; IgG; COVID-19 assay kit
Applicable Applications for COVID-19 assay kit
Immunoassay (IA)
Application Notes
The recomLine SARS-CoV-2 IgG [Aviditat] is a line immunoassay. The separate line-up of individual antigens means that, unlike ELISA, the test allows identification of specific antibodies against the individual an-tigens of the various coronaviruses. The test uses for SARS-CoV-2 (severe acute respiratory syndrome Coronavirus 2) the following re-combinant antigens: NP (nucleocapsid), RBD (receptor binding domain of the spike protein) and S1 (S1 subunit of the spike protein). For the seasonally occurring human coronaviruses (HCoV: NL63, OC43, 229E, HKU1) nucleocapsid proteins (NP) are used.
For the avidity determination of the IgG antibodies, the avidity reagent can be requested separately.
For the avidity determination of the IgG antibodies, the avidity reagent can be requested separately.
Test Principle and Procedure
Highly purified recombinant antigens (NP, RBD and S1 for SARS-CoV-2, as well as NP for NL63, OC43, 229E, HKU1) are fixed to nitrocellu-lose membrane test strips. 1. The test strips are incubated with the diluted serum or plasma sample with specific antibodies binding to the pathogen antigens on the test strips. 2. Unbound antibodies are then washed away. 3. The strips are incubated in a second step with anti-human immu-noglobulin antibodies (IgG) that are coupled to horseradish peroxi-dase. 4. Unbound conjugate antibodies are then washed away. 5. Specific bound antibody is detected in a colour reaction catalysed by the peroxidase. If an antigen-antibody reaction has taken place, a dark band appears on the strip at the corresponding location.
Control bands are located at the top end of the test strip:
a) The reaction control below the strip number for which every se-rum/plasma sample must show a reaction.
b) The conjugate controls (IgG) are used to check the conjugate and strip type used (Ig class specific).
c) 'Cut-off control': The intensity of this band enables an evaluation of the reactivity of the individual antigen bands (see 9.2 Analysis).
Control bands are located at the top end of the test strip:
a) The reaction control below the strip number for which every se-rum/plasma sample must show a reaction.
b) The conjugate controls (IgG) are used to check the conjugate and strip type used (Ig class specific).
c) 'Cut-off control': The intensity of this band enables an evaluation of the reactivity of the individual antigen bands (see 9.2 Analysis).
Product Categories/Family for COVID-19 assay kit
Similar Products
Product Notes
The COVID-19 (Catalog #AAA191649) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's COVID 19 IgG Coronavirus can be used in a range of immunoassay formats including, but not limited to, Immunoassay (IA). The recomLine SARS-CoV-2 IgG [Aviditat] is a line immunoassay. The separate line-up of individual antigens means that, unlike ELISA, the test allows identification of specific antibodies against the individual an-tigens of the various coronaviruses. The test uses for SARS-CoV-2 (severe acute respiratory syndrome Coronavirus 2) the following re-combinant antigens: NP (nucleocapsid), RBD (receptor binding domain of the spike protein) and S1 (S1 subunit of the spike protein). For the seasonally occurring human coronaviruses (HCoV: NL63, OC43, 229E, HKU1) nucleocapsid proteins (NP) are used. For the avidity determination of the IgG antibodies, the avidity reagent can be requested separately. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 IgG Coronavirus, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
