Typical Testing Data/Standard Curve (for reference only)
Human Collagen Type VII ELISA Kit | COL7 elisa kit
Human Collagen Type VII ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: COL-7 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for COL-7. Standards or samples are then added to the microtiter plate wells and COL-7 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of COL-7 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for COL-7 are added to each well to "sandwich" the COL-7 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain COL-7 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL-7 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. In the absence of mutations, however, an acquired form of this disease can result from an autoimmune response made to type VII collagen. [provided by RefSeq, Jul 2008]
Uniprot Description
COL7A1: the alpha chain of type VII collagen, an extra-cellular basement membrane protein restricted to the zone beneath stratified squamous epithelia. Type VII collagen fibrils are composed of three identical alpha collagen chains. Forms anchoring fibrils, which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen. Dystrophic epidermolysis bullosa can result from defects in this protein or an autoimmune response made to type VII collagen. Homotrimer. Interacts with MIA3, facilitating its loading into transport carriers and subsequent secretion. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide; Extracellular matrix; Inhibitor
Chromosomal Location of Human Ortholog: 3p21.1
Cellular Component: extracellular matrix; extracellular space; collagen type VII; endoplasmic reticulum lumen; extracellular region; basement membrane
Molecular Function: serine-type endopeptidase inhibitor activity; identical protein binding; protein binding
Biological Process: extracellular matrix disassembly; collagen catabolic process; epidermis development; extracellular matrix organization and biogenesis; cell adhesion
Disease: Transient Bullous Dermolysis Of The Newborn; Epidermolysis Bullosa Dystrophica, Autosomal Dominant; Nail Disorder, Nonsyndromic Congenital, 8; Epidermolysis Bullosa Dystrophica, Autosomal Recessive; Epidermolysis Bullosa Dystrophica, Pretibial; Epidermolysis Bullosa Pruriginosa; Epidermolysis Bullosa With Congenital Localized Absence Of Skin And Deformity Of Nails
Research Articles on COL7
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Product Notes
The Human COL7 col7a1 (Catalog #AAA723146) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA723146 ELISA Kit recognizes Human COL7. It is sometimes possible for the material contained within the vial of "Collagen Type VII, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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