Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Typical Testing Data/Standard Curve (for reference only)

Sheep Collagen Type VI Alpha 1 ELISA Kit | COL6a1 elisa kit

Sheep Collagen Type VI Alpha 1 ELISA Kit

Gene Names
COL6A1; OPLL
Reactivity
Sheep
Synonyms
Collagen Type VI Alpha 1; Sheep Collagen Type VI Alpha 1 ELISA Kit; COL6a1 elisa kit
Ordering
For Research Use Only!
Reactivity
Sheep
Specificity
This assay has high sensitivity and excellent specificity for detection of COL-6alpha1. No significant cross-reactivity or interference between COL-6alpha1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between COL-6alpha1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for COL6a1 elisa kit
Intended Uses: This COL-6alpha1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep COL-6alpha1. This ELISA kit for research use only!

Principle of the Assay: COL-6alpha1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-COL-6alpha1 antibody and an COL-6alpha1-HRP conjugate. The assay sample and buffer are incubated together with COL-6alpha1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COL-6alpha1 concentration since COL-6alpha1 from samples and COL-6alpha1-HRP conjugate compete for the anti-COL-6alpha1 antibody binding site. Since the number of sites is limited, as more sites are occupied by COL-6alpha1 from the sample, fewer sites are left to bind COL-6alpha1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL-6alpha1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for COL6a1 elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
108,529 Da
NCBI Official Full Name
collagen, type VI, alpha 1, isoform CRA_b, partial
NCBI Official Synonym Full Names
collagen, type VI, alpha 1
NCBI Official Symbol
COL6A1
NCBI Official Synonym Symbols
OPLL
NCBI Protein Information
collagen alpha-1(VI) chain; collagen alpha-1(VI) chain; alpha 1 (VI) chain (61 AA); collagen VI, alpha-1 polypeptide
UniProt Protein Name
Collagen alpha-1(VI) chain
Protein Family
UniProt Gene Name
COL6A1
UniProt Entry Name
CO6A1_HUMAN

NCBI Description

The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively. The protein encoded by this gene is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy. [provided by RefSeq, Jul 2008]

Uniprot Description

COL6A1: Collagen VI acts as a cell-binding protein. Defects in COL6A1 are a cause of Bethlem myopathy (BM). BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles. Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD); also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy. Belongs to the type VI collagen family.

Protein type: Extracellular matrix; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; protein complex; membrane; endoplasmic reticulum lumen; lysosomal membrane; extracellular region; sarcolemma; collagen type VI

Molecular Function: platelet-derived growth factor binding

Biological Process: osteoblast differentiation; collagen catabolic process; axon guidance; extracellular matrix disassembly; extracellular matrix organization and biogenesis; cell adhesion

Disease: Bethlem Myopathy; Ullrich Congenital Muscular Dystrophy

Research Articles on COL6a1

Similar Products

Product Notes

The Sheep COL6a1 col6a1 (Catalog #AAA743010) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA743010 ELISA Kit recognizes Sheep COL6a1. It is sometimes possible for the material contained within the vial of "Collagen Type VI Alpha 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.