anti-Human Collagen IV alpha3 Antibody | anti-COL4A3 antibody

Collagen IV alpha3 (Cleaved-Leu1425) Antibody

Cat. Num. AAA853668

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Collagen IV alpha3; Collagen IV alpha3 (Cleaved-Leu1425) Antibody; Collagen alpha-3(IV) chain; Goodpasture antigen; Tumstatin; CO4A3; anti-COL4A3 antibody

• Reactivity: Human
• Specificity: Collagen IV alpha3 (Cleaved-Leu1425) antibody detects endogenous levels of fragment of activated Collagen IV alpha3 resulting from cleavage adjacent to Leu1425.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1670

• Applicable Applications for anti-COL4A3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized peptide derived from human Collagen IV alpha3.
• Preparation and Storage: Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from293 cells, treated with etoposide (25uM, 1hour), using Collagen IV alpha3 (Cleaved-Leu1425) antibody.)

References

Mariyama M., J. Biol. Chem. 269:23013-23017(1994). Leinonen A., Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases. Heidet L., J. Am. Soc. Nephrol. 12:97-106(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 89142730
• NCBI GeneID: 1285
• NCBI Accession #: NP_000082.2
• NCBI GenBank Nucleotide #: NM_000091.4
• UniProt Accession #: Q01955; Q53QQ1; Q53R14; Q53RW8; Q9BQT2; Q9NYC4; Q9UDJ9; Q9UDK9; Q9UDL0; Q9UDL1
• Molecular Weight: 135,079 Da
• NCBI Official Full Name: collagen alpha-3(IV) chain
• NCBI Official Synonym Full Names: collagen type IV alpha 3 chain
• NCBI Official Symbol: COL4A3
• NCBI Protein Information: collagen alpha-3(IV) chain
• UniProt Protein Name: Collagen alpha-3(IV) chain
• Protein Family: Collagen
• UniProt Gene Name: COL4A3

NCBI Description

Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jun 2010]

Uniprot Description

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.