Goat Collagen Type IV ELISA Kit | COL4 elisa kit

Goat Collagen Type IV ELISA Kit

Cat. Num. AAA738801

• Gene Names: COL4A1; ICH; HANAC; POREN1; arresten
• Reactivity: Goat
• Synonyms: Collagen Type IV; Goat Collagen Type IV ELISA Kit; COL4 elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This COL4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat COL4. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL4 elisa kit

Principle of the Assay: COL4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-COL4 antibody and an COL4-HRP conjugate. The assay sample and buffer are incubated together with COL4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COL4 concentration since COL4 from samples and COL4-HRP conjugate compete for the anti-COL4 antibody binding site. Since the number of sites is limited, as more sites are occupied by COL4 from the sample, fewer sites are left to bind COL4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL4 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for COL4 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 119629513
• NCBI GeneID: 1282
• Molecular Weight: 127,981 Da
• NCBI Official Full Name: collagen, type IV, alpha 1, isoform CRA_a
• NCBI Official Synonym Full Names: collagen, type IV, alpha 1
• NCBI Official Symbol: COL4A1
• NCBI Official Synonym Symbols: ICH; HANAC; POREN1; arresten
• NCBI Protein Information: collagen alpha-1(IV) chain; collagen alpha-1(IV) chain; COL4A1 NC1 domain; collagen IV, alpha-1 polypeptide; collagen of basement membrane, alpha-1 chain
• UniProt Protein Name: Collagen alpha-1(IV) chain
• Protein Family: Collagen type IV alpha-3-binding protein
• UniProt Gene Name: COL4A1
• UniProt Entry Name: CO4A1_HUMAN

NCBI Description

This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jul 2008]

Uniprot Description

COL4A1: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH). Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant. Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC). The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries. Defects in COL4A1 are a cause of familial porencephaly (POREN1). Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles. Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: extracellular matrix; endoplasmic reticulum lumen; collagen type IV; extracellular region; basement membrane

Molecular Function: protein binding; platelet-derived growth factor binding; extracellular matrix structural constituent; extracellular matrix constituent conferring elasticity

Biological Process: patterning of blood vessels; axon guidance; collagen catabolic process; receptor-mediated endocytosis; extracellular matrix disassembly; extracellular matrix organization and biogenesis; epithelial cell differentiation; blood vessel morphogenesis; brain development; neuromuscular junction development

Disease: Porencephaly 1; Brain Small Vessel Disease With Or Without Ocular Anomalies; Angiopathy, Hereditary, With Nephropathy, Aneurysms, And Muscle Cramps; Hemorrhage, Intracerebral, Susceptibility To; Retinal Arteries, Tortuosity Of