anti-Human Collagen III alpha1 Antibody | anti-COL3A1 antibody

Collagen III alpha1 (Cleaved-Gly1221) Antibody

Cat. Num. AAA852986

• Gene Names: COL3A1; EDS4A; EDSVASC
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Collagen III alpha1; Collagen III alpha1 (Cleaved-Gly1221) Antibody; Collagen alpha-1(III) chain [Precursor]; anti-COL3A1 antibody

• Reactivity: Human
• Specificity: Collagen III alpha1 (Cleaved-Gly1221) antibody detects endogenous levels of fragment of activated Collagen III alpha1 resulting from cleavage adjacent to Gly1221.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1466

• Applicable Applications for anti-COL3A1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized peptide derived from human Collagen III alpha1.
• Preparation and Storage: Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from A549 cells, treated with etoposide (25uM, 24hours), using Collagen III alpha1 (Cleaved-Gly1221) antibody.)

References

Ala-Kokko L., Biochem. J. 260:509-516(1989). Valkkila M., Matrix Biol. 20:357-366(2001). Hillier L.W., Nature 434:724-731(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 4502951
• NCBI GeneID: 1281
• NCBI Accession #: NP_000081.1
• NCBI GenBank Nucleotide #: NM_000090.3
• UniProt Accession #: P02461; P78429; Q15112; Q16403; Q53S91; Q541P8; Q6LDB3; Q6LDJ2; Q6LDJ3; Q7KZ56; D2JYH5; D3DPH4
• Molecular Weight: 111,907 Da
• NCBI Official Full Name: collagen alpha-1(III) chain preproprotein
• NCBI Official Synonym Full Names: collagen type III alpha 1 chain
• NCBI Official Symbol: COL3A1
• NCBI Official Synonym Symbols: EDS4A; EDSVASC
• NCBI Protein Information: collagen alpha-1(III) chain
• UniProt Protein Name: Collagen alpha-1(III) chain
• Protein Family: Collagen
• UniProt Gene Name: COL3A1

NCBI Description

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]

Uniprot Description

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.