Human Collagen Type II ELISA Kit | COL2 elisa kit

Human Collagen Type II ELISA Kit

Cat. Num. AAA765936

• Gene Names: COL2A1; AOM; ANFH; SEDC; STL1; COL11A3
• Reactivity: Human
• Synonyms: Collagen Type II; Human Collagen Type II ELISA Kit; COL2 (Collagen Type II)/Chondrocalcin/COL2A1/SEDC/Alpha-1 type II collagen/ANFH/AOM/cartilage collagen/COL11A3/Collagen 2/collagen alpha-1 (II) chain/collagen II; alpha-1 polypeptide/collagen type 2; COL2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of COL2. No significant cross-reactivity or interference between COL2 and analogues was observed.
• Sequence Length: 1487

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 46.875-3000pg/ml
• Sensitivity: 28.125pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 119578374
• NCBI GeneID: 1280
• Molecular Weight: 29,781 Da
• NCBI Official Full Name: collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital), isoform CRA_e
• NCBI Official Synonym Full Names: collagen type II alpha 1
• NCBI Official Symbol: COL2A1
• NCBI Official Synonym Symbols: AOM; ANFH; SEDC; STL1; COL11A3
• NCBI Protein Information: collagen alpha-1(II) chain
• UniProt Protein Name: Collagen alpha-1(II) chain
• Protein Family: Zinc finger protein CONSTANS
• UniProt Gene Name: COL2A1
• UniProt Entry Name: CO2A1_HUMAN

NCBI Description

This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

COL2A1: the alpha-1 chain of type II collagen, an extra-cellular matrix protein found in cartilage and the vitreous humor of the eye. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Chondrocalcin is the calcium binding C-propeptide of this collagen molecule. Defects in this protein are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. There are two transcripts identified for this gene. Belongs to the fibrillar collagen family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13.11

Cellular Component: basement membrane; collagen type II; endoplasmic reticulum lumen; extracellular matrix; extracellular region; extracellular space

Molecular Function: extracellular matrix structural constituent conferring tensile strength; identical protein binding; metal ion binding; platelet-derived growth factor binding

Biological Process: axon guidance; cartilage condensation; cartilage development; central nervous system development; chondrocyte differentiation; collagen catabolic process; collagen fibril organization; endochondral ossification; extracellular matrix disassembly; extracellular matrix organization and biogenesis; heart morphogenesis; inner ear morphogenesis; limb bud formation; notochord development; palate development; proteoglycan metabolic process; regulation of gene expression; regulation of immune response; sensory perception of sound; skeletal development; tissue homeostasis; visual perception

Disease: Achondrogenesis, Type Ii; Avascular Necrosis Of Femoral Head, Primary; Czech Dysplasia; Epiphyseal Dysplasia, Multiple, With Myopia And Conductive Deafness; Kniest Dysplasia; Legg-calve-perthes Disease; Osteoarthritis With Mild Chondrodysplasia; Otospondylomegaepiphyseal Dysplasia; Platyspondylic Lethal Skeletal Dysplasia, Torrance Type; Spondyloepimetaphyseal Dysplasia, Strudwick Type; Spondyloepiphyseal Dysplasia Congenita; Spondyloepiphyseal Dysplasia, Stanescu Type; Spondyloperipheral Dysplasia; Stickler Syndrome, Type I; Stickler Syndrome, Type I, Nonsyndromic Ocular