Collagen Type XV (COL15) Recombinant Protein | COL15 recombinant protein

Recombinant Collagen Type XV (COL15)

Cat. Num. AAA2011530

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Collagen Type XV (COL15); Recombinant Collagen Type XV (COL15); COL15 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-NYEKPALHL AALNMPFSGD IRADFQCFKQ ARAAGLLSTY RAFLSSHLQD LSTIVRKAER YSLPIVNLKG QVLFNNWDSI FSGHGGQFNM HIPIYSFDGR DIMTDPSWPQ KVIWHGSSPH GVRLVDNYCE AWRTADTAVT GLASPLSTGK ILDQKAYSCA NRLIVLCIEN SFMTDARK
• Sequence Length: 1388

• Applicable Applications for COL15 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Asn1212~Lys1388 (Accession # P39059) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 116008152
• NCBI GeneID: 1306
• NCBI Accession #: NP_001846.3
• NCBI GenBank Nucleotide #: NM_001855.4
• UniProt Accession #: P39059; Q5T6J4; Q9UDC5; Q9Y4W4
• Molecular Weight: 21.3kDa
• NCBI Official Full Name: collagen alpha-1(XV) chain
• NCBI Official Synonym Full Names: collagen, type XV, alpha 1
• NCBI Official Symbol: COL15A1
• NCBI Protein Information: collagen alpha-1(XV) chain; collagen alpha-1(XV) chain; restin; endostatin-XV; collagen type XV proteoglycan; collagen XV, alpha-1 polypeptide
• UniProt Protein Name: Collagen alpha-1(XV) chain
• Protein Family: Zinc finger protein CONSTANS
• UniProt Gene Name: COL15A1
• UniProt Entry Name: COFA1_HUMAN

NCBI Description

This gene encodes the alpha chain of type XV collagen, a member of the FACIT collagen family (fibril-associated collagens with interrupted helices). Type XV collagen has a wide tissue distribution but the strongest expression is localized to basement membrane zones so it may function to adhere basement membranes to underlying connective tissue stroma. The proteolytically produced C-terminal fragment of type XV collagen is restin, a potentially antiangiogenic protein that is closely related to endostatin. Mouse studies have shown that collagen XV deficiency is associated with muscle and microvessel deterioration. [provided by RefSeq, May 2013]

Uniprot Description

COL15A1: Structural protein that stabilizes microvessels and muscle cells, both in heart and in skeletal muscle. Belongs to the multiplexin collagen family.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix

Chromosomal Location of Human Ortholog: 9q21-q22

Cellular Component: extracellular matrix; extracellular space; endoplasmic reticulum lumen; collagen type XV; integral to membrane; extracellular region

Molecular Function: extracellular matrix structural constituent

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; angiogenesis; cell differentiation; signal transduction; cell adhesion